Webinar: ELISA Principles & Key Considerations for Reliable Results

ELISA (Enzyme-Linked Immunosorbent Assay) is a highly sensitive and versatile immunoassay technique that combines the specificity of antigen-antibody interactions with the efficiency of enzymatic catalysis. This method offers various formats, including sandwich, competitive, and indirect assays, each tailored to specific applications such as antibody detection and small molecule analysis. Key operational considerations involve meticulous sample handling to prevent contamination, precise control of incubation conditions, thorough yet gentle washing to minimize nonspecific binding, and accurate timing during substrate addition and reaction termination. All in all, ELISA provides a robust platform for diverse research needs. Its reliability and versatility make it an essential tool in advancing scientific discovery, ensuring accurate and reproducible results.