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Enzyme-linked immunosorbent assay (ELISA), originating in the 1970s, is based on the principle that soluble antigens or antibodies are specifically bound to solid-phase carriers such as polystyrene. By combining specific recognition between antigens and antibodies with efficient enzyme catalysis, the color reaction of the substrate and its color depth are used to achieve qualitative or quantitative analysis of immune responses. With simple operation, high sensitivity, specificity, and high throughput, it can be widely used in life science, clinical diagnosis, environmental monitoring, drug residue detection, and so on.
CUSABIO ELISA kit relies on years of research and development experience and an independent platform, using multi-dimensional quality control, to develop ready-to-use, high-quality, and high-performance ELISA kits. This webinar will provide a comprehensive overview of ELISA techniques, focusing on both theoretical and practical aspects. We will start with an introduction to the fundamental principles of ELISA, followed by detailed discussions on developing and processing standard curves for quantitative ELISA. Additionally, we will cover methods for analyzing qualitative ELISA data. The session will conclude with common challenges encountered during standard curve development and effective strategies to overcome them.
1. Introduction to ELISA principles
2. Development and data processing of standard curves for quantitative ELISA
3. Data analysis for qualitative ELISA
4. Common problems in standard curve development and their solutions
Ying Zhou PhD
Product Manager, CUSABIO
Dr. Ying Zhou is a Product Manager at CUSABIO, responsible for product marketing, development, and optimization. Before joining CUSABIO, Dr. Zhou earned her Ph.D. in Biology and Biotechnology from Worcester Polytechnic Institute in the United States. Following her graduation, she pursued post-doctoral training at the University of California, Davis. Dr. Zhou's expertise lies in molecular biology and protein science, specifically in mRNA expression and regulation. She is particularly interested in mRNA stability and RNA-interacting proteins.
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