Human apolipoprotein A1 , Apo-A1 ELISA Kit Bovine Apolipoprotein B , APOB ELISA Kit Chicken Apolipoprotein B , APOB ELISA Kit Mouse Apolipoprotein B , APOB ELISA Kit Pig Apolipoprotein B , APOB ELISA Kit Chicken Apolipoprotein E , APOE ELISA Kit Dog Apolipoprotein E , APOE ELISA Kit Human apolipoprotein E , Apo-E ELISA Kit Mouse apolipoprotein E , Apo-E ELISA Kit Rat apolipoprotein E , Apo-E ELISA Kit Sheep Apolipoprotein E , APOE ELISA Kit Bovine Clusterin , CLU ELISA Kit Chicken Clusterin , CLU ELISA Kit Rabbit Clusterin , CLU ELISA Kit Sheep Clusterin , CLU ELISA Kit
Donkey Estradiol , E2 ELISA Kit Duck estradiol , E2 ELISA Kit Sheep Estradiol , E2 ELISA Kit Sheep Follicle Stimulating Hormone , FSH ELISA Kit Human luteinizing hormone , LH ELISA Kit Monkey luteinizing hormone , LH ELISA Kit Sheep Luteinizing Hormone , LH ELISA Kit Human Prolactin/Luteotropic Hormone , PRL/LTH ELISA Kit Donkey Progesterone , PROG ELISA Kit Duck progesterone , PROG ELISA Kit Canine Testosterone , T ELISA Kit Chicken Testosterone , T ELISA Kit Fish Testosterone , T ELISA Kit Goat Testosterone , T ELISA Kit Sheep Testosterone , T ELISA Kit
Bovine free tri-iodothyronine indes , FT3 ELISA Kit Canine Free Tri-iodothyronine Indes , Free-T3 ELISA Kit Chicken free tri-iodothyronine indes , FT3 ELISA Kit Sheep free tri-iodothyronine indes , FT3 ELISA Kit Fish free thyroxine , FT4 ELISA Kit Pig free thyroxine , FT4 ELISA Kit Horse tri-iodothyronine , T3 ELISA Kit Monkey tri-iodothyronine , T3 ELISA Kit Sheep tri-iodothyronine , T3 ELISA Kit Cat thyroxine , T4 ELISA Kit Chicken thyroxine , T4 ELISA Kit Goat Thyroxine , T4 ELISA Kit Horse thyroxine , T4 ELISA Kit Pig Thyroxine , T4 ELISA Kit Sheep thyroxine , T4 ELISA Kit
Please scan for our contact information
- ELISA Kit
- Human anti-Mullerian hormone , AMH ELISA Kit
- Anti-Mullerian hormone is a member of the transforming growth factor-beta gene family which mediates male sexual differentiation. Anti-Mullerian hormone causes the regression of Mullerian ducts which would otherwise differentiate into the uterus and fallopian tubes. Some mutations in the anti-Mullerian hormone result in persistent Mullerian duct syndrome. [provided by RefSeq]
Human anti-Mullerian hormone , AMH ELISA Kit
||MIF, MIS, Mullerian inhibiting factor|Mullerian inhibiting substance|
|other species:||Dog Sheep Rat Bovine Mouse Pig|
||0.375 ng/ml-150 ng/ml|
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
|Specificty:||This assay has high sensitivity and excellent specificity for detection of Human AMH. No significant cross-reactivity or interference between Human AMH and analogues was observed.|
|Precision:||Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
|Sample collection and storage:||Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
|Assay procedure:||Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
|Calculation of results:||Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the AMH concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
|Title||Effects of dehydroepiandrosterone on in vivo ovine follicular development|
|Author||Narkwichean A et al|
|Type of publication||Product-specific|
|Other information||2013 Nov 20. [Epub ahead of print]|