Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human GPRC5D at 5 μg/mL can bind Anti-GPRC5D recombinant antibody (CSB-RA882153MA01HU), the EC50 is 8.847-10.38 ng/mL.
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
1-345aa
Target Protein Sequence
MYKDCIESTGDYFLLCDAEGPWGIILESLAILGIVVTILLLLAFLFLMRKIQDCSQWNVLPTQLLFLLSVLGLFGLAFAFIIELNQQTAPVRYFLFGVLFALCFSCLLAHASNLVKLVRGCVSFSWTTILCIAIGCSLLQIIIATEYVTLIMTRGMMFVNMTPCQLNVDFVVLLVYVLFLMALTFFVSKATFCGPCENWKQHGRLIFITVLFSIIIWVVWISMLLRGNPQFQRQPQWDDPVVCIALVTNAWVFLLLYIVPELCILYRSCRQECPLQGNACPVTAYQHSFQVENQELSRARDSDGAEEDVALTSYGTPIQPQTVDPTQECFIPQAKLSPQQDAGGV
Protein Length
Full Length
Tag Info
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
Form
Lyophilized powder
Note: We will default ship it in lyophilized form with normal bule ice packs. However, if you request to ship in liquid form, it needs to be shipped with dry ice, please communicate with us in advance and extra fees for dry ice and dry ice box will be charged.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Our cutting-edge Recombinant Human GPRC5D is a vital component in the field of signal transduction research. GPRC5D, short for G-protein coupled receptor family C group 5 member D, is an integral part of the G-protein coupled receptor family and plays a significant role in cellular signaling processes. This recombinant protein is derived from a mammalian cell expression system, ensuring its authenticity and functionality in human systems. It covers the full length of the GPRC5D protein, spanning amino acids 1 to 345. To facilitate easy detection and purification, it features a C-terminal 10xHis tag. Additionally, the endotoxin level is meticulously controlled, with a concentration of less than 1.0 EU/μg as determined by the LAL method, guaranteeing the utmost purity and reliability in your experiments.
The activity of this Recombinant Human GPRC5D can be assessed through its binding ability in a functional ELISA. When immobilized at 5 μg/mL, it exhibits a strong binding affinity for the Anti-GPRC5D recombinant antibody (CSB-RA882153MA01HU), with an EC50 range of 8.847-10.38 ng/mL. This indicates its biological functionality and enables precise measurements in your experimental assays. For your convenience, the Recombinant Human GPRC5D is provided in lyophilized powder form, ensuring stability and ease of storage. Embrace the potential of this remarkable protein in unraveling the intricate mechanisms of signal transduction and advancing your research endeavors.
