Recombinant Human G-protein coupled receptor family C group 5 member D(GPRC5D)-VLPs (Active)

In Stock
Code CSB-MP882153HU
Size How to order?
Image
  • CSB-MP882153HU is detected by Mouse anti-6*His monoclonal antibody.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized Human GPRC5D at 5 μg/mL can bind Anti-GPRC5D recombinant antibody (CSB-RA882153MA01HU), the EC50 is 8.847-10.38 ng/mL. Biological Activity Assay
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Description

This recombinant human GPRC5D protein is generated by the HEK293 cells expression systemand is expressed with sequence Met1-Val345 of human GPRC5D fused with a 10xHis-tag at the C-terminal. It is a virus-like particle. Its endotoxin and biological activity have been measured.

GPRC5D is highly expressed on the surface of primary multiple myeloma (MM) cells, while its expression in normal tissues is limited to the hair follicle. GPRC5D thus becomes an attractive target for the treatment of MM and is expected to provide new therapies for MM patients with poor prognoses. The treatment strategy of GPRC5D in MM mainly focuses on GPRC5D-CAR T cell therapy and GPRC5D-combined with bispecific antibody therapy. Currently, there are several combination drugs targeting GPRC5D or based on GPRC5D in clinical development. For example, Talquetamab (JNJ-64407564) is a GPRC5D/CD3 bispecific antibody that inhibits tumor growth by recruiting and activating CD3-positive T cells and inducing T cells to kill GPRC5D-positive MM cells.

Endotoxin Less than 1.0 EU/ug as determined by LAL method.
Activity Measured by its binding ability in a functional ELISA. Immobilized Human GPRC5D at 5 μg/mL can bind Anti-GPRC5D recombinant antibody (CSB-RA882153MA01HU), the EC50 is 8.847-10.38 ng/mL.
Target Names GPRC5D
Uniprot No. Q9NZD1
Molecular Characterization
Species Homo sapiens (Human)
Source Mammalian cell
Expression Region 1-345aa
Target Protein Sequence MYKDCIESTGDYFLLCDAEGPWGIILESLAILGIVVTILLLLAFLFLMRKIQDCSQWNVLPTQLLFLLSVLGLFGLAFAFIIELNQQTAPVRYFLFGVLFALCFSCLLAHASNLVKLVRGCVSFSWTTILCIAIGCSLLQIIIATEYVTLIMTRGMMFVNMTPCQLNVDFVVLLVYVLFLMALTFFVSKATFCGPCENWKQHGRLIFITVLFSIIIWVVWISMLLRGNPQFQRQPQWDDPVVCIALVTNAWVFLLLYIVPELCILYRSCRQECPLQGNACPVTAYQHSFQVENQELSRARDSDGAEEDVALTSYGTPIQPQTVDPTQECFIPQAKLSPQQDAGGV
Mol. Weight 40.6 kDa
Protein Length Full Length
Tag Info C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
Form Lyophilized powder
Note: We will default ship it in lyophilized form with normal bule ice packs. However, if you request to ship in liquid form, it needs to be shipped with dry ice, please communicate with us in advance and extra fees for dry ice and dry ice box will be charged.
Buffer Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Gene References into Functions
  1. GPRC5D in MM cells makes this gene and its encoded surface protein as promising markers for monitoring the tumor load and hopefully also as targets for antimyeloma antibodies. PMID: 23510526
  2. Overexpression of G protein-coupled receptor 5D in the bone marrow is associated with poor prognosis in patients with multiple myeloma. PMID: 22591013
Subcellular Location Cell membrane; Multi-pass membrane protein.
Protein Families G-protein coupled receptor 3 family
Tissue Specificity Widely expressed in the peripheral system. Expression pattern is high in pancreas, medium in kidney, small intestine, spleen and testis, low in lung, colon, leukocyte, prostate and thymus and not detectable in brain, heart, liver, placenta, skeletal muscl
Database Links

HGNC: 13310

OMIM: 607437

KEGG: hsa:55507

STRING: 9606.ENSP00000228887

UniGene: Hs.644599

Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
Address
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
Join Us with

Subscribe newsletter

Leave a message

© 2007-2022 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1