Product Details

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human GPRC5D at 5 μg/mL can bind Anti-GPRC5D recombinant antibody (CSB-RA882153MA01HU), the EC₅₀ is 8.847-10.38 ng/mL.

Target Names

GPRC5D

Uniprot No.

Q9NZD1

Molecular Characterization

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

1-345aa

Target Protein Sequence

MYKDCIESTGDYFLLCDAEGPWGIILESLAILGIVVTILLLLAFLFLMRKIQDCSQWNVLPTQLLFLLSVLGLFGLAFAFIIELNQQTAPVRYFLFGVLFALCFSCLLAHASNLVKLVRGCVSFSWTTILCIAIGCSLLQIIIATEYVTLIMTRGMMFVNMTPCQLNVDFVVLLVYVLFLMALTFFVSKATFCGPCENWKQHGRLIFITVLFSIIIWVVWISMLLRGNPQFQRQPQWDDPVVCIALVTNAWVFLLLYIVPELCILYRSCRQECPLQGNACPVTAYQHSFQVENQELSRARDSDGAEEDVALTSYGTPIQPQTVDPTQECFIPQAKLSPQQDAGGV

Mol. Weight

40.6 kDa

Protein Length

Full Length

Tag Info

C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)

Form

Lyophilized powder
Note: We will default ship it in lyophilized form with normal bule ice packs. However, if you request to ship in liquid form, it needs to be shipped with dry ice, please communicate with us in advance and extra fees for dry ice and dry ice box will be charged.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Our cutting-edge Recombinant Human GPRC5D is a vital component in the field of signal transduction research. GPRC5D, short for G-protein coupled receptor family C group 5 member D, is an integral part of the G-protein coupled receptor family and plays a significant role in cellular signaling processes. This recombinant protein is derived from a mammalian cell expression system, ensuring its authenticity and functionality in human systems. It covers the full length of the GPRC5D protein, spanning amino acids 1 to 345. To facilitate easy detection and purification, it features a C-terminal 10xHis tag. Additionally, the endotoxin level is meticulously controlled, with a concentration of less than 1.0 EU/μg as determined by the LAL method, guaranteeing the utmost purity and reliability in your experiments.

The activity of this Recombinant Human GPRC5D can be assessed through its binding ability in a functional ELISA. When immobilized at 5 μg/mL, it exhibits a strong binding affinity for the Anti-GPRC5D recombinant antibody (CSB-RA882153MA01HU), with an EC50 range of 8.847-10.38 ng/mL. This indicates its biological functionality and enables precise measurements in your experimental assays. For your convenience, the Recombinant Human GPRC5D is provided in lyophilized powder form, ensuring stability and ease of storage. Embrace the potential of this remarkable protein in unraveling the intricate mechanisms of signal transduction and advancing your research endeavors.

Target Background

Gene References into Functions

GPRC5D in MM cells makes this gene and its encoded surface protein as promising markers for monitoring the tumor load and hopefully also as targets for antimyeloma antibodies. PMID: 23510526
Overexpression of G protein-coupled receptor 5D in the bone marrow is associated with poor prognosis in patients with multiple myeloma. PMID: 22591013

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 3 family

Tissue Specificity

Widely expressed in the peripheral system. Expression pattern is high in pancreas, medium in kidney, small intestine, spleen and testis, low in lung, colon, leukocyte, prostate and thymus and not detectable in brain, heart, liver, placenta, skeletal muscl

Database Links

HGNC: 13310

OMIM: 607437

KEGG: hsa:55507

STRING: 9606.ENSP00000228887

UniGene: Hs.644599

Code	CSB-MP882153HU
Abbreviation	Recombinant Human GPRC5D protein-VLPs (Active)
MSDS	MSDS Datasheet
Size	$570
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Image	CSB-MP882153HU is detected by Mouse anti-6*His monoclonal antibody. Activity Measured by its binding ability in a functional ELISA. Immobilized Human GPRC5D at 5 μg/mL can bind Anti-GPRC5D recombinant antibody (CSB-RA882153MA01HU), the EC₅₀ is 8.847-10.38 ng/mL. Biological Activity Assay
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Recombinant Human G-protein coupled receptor family C group 5 member D (GPRC5D)-VLPs (Active)

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