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This Human Claudin-4 (CLDN4) recombinant protein was produced in mammalian cell, where the gene sequence encoding human CLDN4 (1-209aa) was expressed with the C-terminal 10xHis tag. The activity was validated.
Both CLDN4 and CLDN8 are considered to be cation barriers and anion channels. We found that catheter-specific knockout of CLDN4 caused hypotension, hypochloremia, metabolic alkalosis, and renal failure symptoms of Na+ and Cl−, and overexpression of CLDN4 resulted in cation-selective MDCK II and anion-selective LLC-PK1 TER was increased in cells, and a decrease in Na+ permeability was observed in MDCK II cells.
|Endotoxin||Less than 1.0 EU/ug as determined by LAL method.|
|Activity||Measured by its binding ability in a functional ELISA. Immobilized Human CLDN4 at 5 μg/mL can bind Anti-CLDN4 recombinant antibody (CSB-RA005506MA1HU), the EC50 is 29.56-50.75 ng/mL.|
(Clostridium perfringens enterotoxin receptor)(CPE-R)(CPE-receptor)(Williams-Beuren syndrome chromosomal region 8 protein)
|Species||Homo sapiens (Human)|
|Target Protein Sequence||MASMGLQVMGIALAVLGWLAVMLCCALPMWRVTAFIGSNIVTSQTIWEGLWMNCVVQSTGQMQCKVYDSLLALPQDLQAARALVIISIIVAALGVLLSVVGGKCTNCLEDESAKAKTMIVAGVVFLLAGLMVIVPVSWTAHNIIQDFYNPLVASGQKREMGASLYVGWAASGLLLLGGGLLCCNCPPRTDKPYSAKYSAARSAAASNYV|
|Mol. Weight||23.4 kDa
|Protein Length||Full Length|
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
|Buffer||Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4|
|Reconstitution||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.|
|Storage Condition||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.|
|Shelf Life||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
|Lead Time||Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.|
|Notes||Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.|
|Datasheet & COA||Please contact us to get it.|
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Channel-forming tight junction protein that mediates paracellular chloride transport in the kidney. Plays a critical role in the paracellular reabsorption of filtered chloride in the kidney collecting ducts. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
|Gene References into Functions||
|Involvement in disease||CLDN4 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.|
|Subcellular Location||Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.|
|Protein Families||Claudin family|