AACS Antibody

Datasheet

Code	CSB-PA001012LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: HEK293 whole cell lysate All lanes: AACS antibody at 2.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76, 68, 31 kDa Observed band size: 76 kDa Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA001012LA01HU at dilution of 1:100 Immunoprecipitating AACS in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA001012LA01HU in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA001012LA01HU (8µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (10µg)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) AACS Polyclonal antibody

Uniprot No.

Q86V21

Target Names

AACS

Alternative Names

AACS antibody; ACSF1Acetoacetyl-CoA synthetase antibody; EC 6.2.1.16 antibody; Acyl-CoA synthetase family member 1 antibody; Protein sur-5 homolog antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Acetoacetyl-CoA synthetase protein (435-598AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The AACS Antibody (Product code: CSB-PA001012LA01HU) is Non-conjugated. For AACS Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA001012LB01HU	AACS Antibody, HRP conjugated	ELISA
FITC	CSB-PA001012LC01HU	AACS Antibody, FITC conjugated
Biotin	CSB-PA001012LD01HU	AACS Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development.

Gene References into Functions

the human AACS promoter is a PPARgamma target gene and this nuclear receptor is recruited to the AACS promoter by direct interaction with Sp1 PMID: 20102333

Subcellular Location

Cytoplasm, cytosol.

Protein Families

ATP-dependent AMP-binding enzyme family

Tissue Specificity

Highly expressed in kidney, heart and brain, but low in liver.

Database Links

HGNC: 21298

OMIM: 614364

KEGG: hsa:65985

STRING: 9606.ENSP00000324842

UniGene: Hs.656073

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Protein Service

Gene Synthesis Service

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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