All
  • All
  • ELISA Kit
  • Protein
  • Antibody
  • Molecular Biology Product
  • Small Molecule
  • Peptide
All
  • All
  • ELISA Kit
  • Protein
  • Antibody
  • Molecular Biology Product
  • Small Molecule
  • Peptide

AAK1 Antibody

Datasheet
Code CSB-PA183703
Size US$297
Order now
Image

  • Gel: 10%SDS-PAGE Lysate: 30 ug Human fetal brain tissue lysate Primary antibody: 1/500 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 30 seconds

  • The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 31024 (AAK1 Antibody) at dilution 1/30, on the right is treated with the fusion protein.
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) AAK1 Polyclonal antibody
Uniprot No.
Q2M2I8
Target Names
AAK1
Alternative Names
AAK 1 antibody; Aak1 antibody; AAK1_HUMAN antibody; Adaptor associated kinase 1 antibody; Adaptor-associated kinase 1 antibody; AP2 associated kinase 1 antibody; AP2 associated protein kinase 1 antibody; AP2-associated protein kinase 1 antibody; DKFZp686F03202 antibody; DKFZp686K16132 antibody; FLJ23712 antibody; FLJ25931 antibody; FLJ31060 antibody; FLJ42882 antibody; FLJ45252 antibody; KIAA1048 antibody; MGC138170 antibody; MGC164568 antibody; MGC164570 antibody
Raised in
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Fusion protein corresponding to N terminal 236 amino acids of Human Adaptor-associated protein kinase 1
Immunogen Species
Homo sapiens (Human)
Clonality
Polyclonal
Concentration
It differs from different batches. Please contact us to confirm it.
Form
Supplied at 1.1mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
Tested Applications
ELISA,WB,IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:1000
IHC 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

AAK1 Antibodies

AAK1 Proteins

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Preferentially, may phosphorylate substrates on threonine residues. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.; (Microbial infection) By regulating clathrin-mediated endocytosis, AAK1 plays a role in the entry of hepatitis C virus as well as for the lifecycle of other viruses such as Ebola and Dengue.
Gene References into Functions
  1. Results present the first structures of AAK1 and BIKE which reveal that all members of the Numb-associated kinase family share unusual activation segment architecture. PMID: 26853940
  2. Two of these kinases that were classified as resistors were PX domain-containing serine/threonine kinase (PXK) and AP2-associated kinase 1 (AAK1), which promote receptor endocytosis and may enable cells to resist TRAIL-induced apoptosis PMID: 25852190
  3. AAK1 and GAK are critical regulators of HCV entry that function in part by activating EGFR, AP2M1, and NUMB, and as the molecular targets underlying the antiviral effect of sunitinib and erlotinib, respectively. PMID: 25653444
  4. AAK1 increases the localization of activated Notch to Rab5-positive endocytic vesicles, while AAK1 depletion or overexpression of Numb, an inhibitor of the pathway, interferes with this localization. PMID: 21464124
  5. These observations suggest that AAK1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its rapid recycling back to the plasma membrane from early/sorting endosome. PMID: 17494869

Show More

Hide All

Subcellular Location
Cell membrane; Peripheral membrane protein. Membrane, clathrin-coated pit. Cell junction, synapse, presynapse.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Tissue Specificity
Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain.
Database Links

HGNC: 19679

OMIM: 616405

KEGG: hsa:22848

STRING: 9606.ENSP00000386456

UniGene: Hs.468878

Promotions
Reward For Reviews
Free antibody trial
Contact Us

  Email: [email protected]
  Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Related Articles
Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?
CUSABIO guaranteed quality
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of email
Email
[email protected]
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2025 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
Privacy Policy
Place an order now

I. Product details

*
*
*
*

II. Contact details

*
*

III. Ship To

*
*
*
*
*
*
*

IV. Bill To

*
*
*
*
*
*
*
*