CYB5B Antibody

Datasheet

Code	CSB-PA006310LA01HU
Size	US$166
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Image	Western blot All lanes: CYB5B antibody at 0.8µg/ml Lane 1: HepG2 whole cell lysate Lane 2: Mouse liver tissue Lane 3: Mouse heart tissue Lane 4: Hela whole cell lysate Lane 5: U87 whole cell lysate Lane 6: EC109 whole cell lysate Lane 7: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 17 kDa Observed band size: 17 kDa Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA006310LA01HU at dilution of 1:100 Immunofluorescent analysis of HepG2 cells using CSB-PA006310LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CYB5B Polyclonal antibody

Uniprot No.

O43169

Target Names

CYB5B

Alternative Names

CYB5B; CYB5M; OMB5; Cytochrome b5 type B; Cytochrome b5 outer mitochondrial membrane isoform

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Cytochrome b5 type B protein (2-118AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CYB5B Antibody (Product code: CSB-PA006310LA01HU) is Non-conjugated. For CYB5B Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA006310LB01HU	CYB5B Antibody, HRP conjugated	ELISA
FITC	CSB-PA006310LC01HU	CYB5B Antibody, FITC conjugated
Biotin	CSB-PA006310LD01HU	CYB5B Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases.

Gene References into Functions

comparison of X-ray crystal structures of human and rat CYB5B reveals a striking difference in packing involving the five-strand beta-sheet, which can be attributed to fully buried residue 21 in strand beta4 PMID: 21574570
The 21 kDa protein overexpressed in Hodgkin Lymphoma and aggressive non-Hodgkin Lymphomas is identical to CYB5B. PMID: 20100355
could be involved in the differential modulation of 17 alpha-hydroxylase and 17,20-lyase activities of P450c17 [type2 cyt-b5] PMID: 11867265
microsomal cytochrome b5 interacts with neuronal calcium sensor protein visinin-like protein-3 in a Ca2+-dependent manner PMID: 14739275

Subcellular Location

Mitochondrion outer membrane.

Protein Families

Cytochrome b5 family

Database Links

HGNC: 24374

OMIM: 611964

KEGG: hsa:80777

STRING: 9606.ENSP00000308430

UniGene: Hs.461131

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Service

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Antibody Service

Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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