HMGB3 Antibody

Datasheet

Code	CSB-PA121578
Size	US$166
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Image	The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA121578(HMGB3 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA121578(HMGB3 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200) Gel: 10%SDS-PAGE, Lysate: 30 μg, Lane 1-4: 293T cells, hela cells, mouse lung tissue, Mouse brain tissue, Primary antibody: CSB-PA121578(HMGB3 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1.5 minutes
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Product Details
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Target Background

Product Details

Uniprot No.

O15347

Target Names

HMGB3

Alternative Names

chromosomal protein; Nonhistone; HMG4 antibody; High mobility group (nonhistone chromosomal) protein 4 antibody; High mobility group box 3 antibody; High mobility group protein 2a antibody; High mobility group protein 4 antibody; High mobility group protein B3 antibody; High mobility group protein HMG4 antibody; HMG 4 antibody; HMG-2a antibody; HMG-4 antibody; HMG2A antibody; HMGB 3 antibody; Hmgb3 antibody; HMGB3_HUMAN antibody; MGC90319 antibody; Non histone chromosomal protein antibody; Nonhistone chromosomal protein HMG4 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse

Immunogen

Synthetic peptide of Human HMGB3

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:1000-1:10000
WB	1:500-1:2000
IHC	1:20-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters. Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor. Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling.

Gene References into Functions

HMGB3 is involved in malignant transformation of hepatocytes and could be a useful biomarker for diagnosis and a potential target for therapy of liver cancer. PMID: 30166860
The study strongly suggests that HMGB3 promotes GBM oncogenesis through the MAPK signalling pathway while miR-200b-3p and miR-200c-3p inhibit its expression. PMID: 30232806
Luciferase reporter assays showed that HMGB3 was directly regulated by miR-205-5p in prostate cancer cells. PMID: 29196733
HMGB3 regulates the genes expression of WNT/beta-catenin pathway PMID: 28678825
Therefore, we infer that HMGB3 is a functional target of miR-27b in modulation of tamoxifen resistance and EMT. PMID: 27363334
these findings demonstrate that HMBG3 may be a potential molecular marker for predicting the prognosis of esophageal squamous cell carcinoma patients PMID: 25755721
our data suggests HMGB3 may serve as an important oncoprotein and indicate that overexpression of HMGB3 in urinary bladder cancer could be used as a potential prognostic marker. PMID: 25647262
High expression of HMGB3 is associated with lymph node metastases in gastric cancer. PMID: 25095979
In this family, microphthalmia, microcephaly, intellectual disability, and short stature are associated with a mutation on the X chromosome in the HMGB3 gene. PMID: 24993872
regulation of HMGB3 by miR-205 reduced both proliferation and invasion of breast cancer cells. PMID: 24098490
Association between co-expression networks involving HMGB3 and differentially-expressed genes in gastric cancer. PMID: 24338397
HMGB3 has a role in gastric cancer disease onset and progression by regulating the cell cycle PMID: 23326140
HMGB3 plays an important role in non-small cell lung cancer progression. PMID: 23609034
Data indicate that myomiR-206 modulates Hmgb3 expression during myogenesis. PMID: 22912879

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Involvement in disease

Microphthalmia, syndromic, 13 (MCOPS13)

Subcellular Location

Nucleus. Chromosome. Cytoplasm.

Protein Families

HMGB family

Tissue Specificity

Expressed predominantly in placenta.

Database Links

HGNC: 5004

OMIM: 300193

KEGG: hsa:3149

STRING: 9606.ENSP00000359393

UniGene: Hs.19114

Promotions

Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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