Recombinant Human High mobility group protein B3 (HMGB3)

Code CSB-YP010561HU
MSDS
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Source Yeast
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Code CSB-EP010561HU
MSDS
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Source E.coli
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Code CSB-EP010561HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010561HU
MSDS
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Source Baculovirus
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Code CSB-MP010561HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HMGB3
Uniprot No.
Alternative Names
chromosomal protein; Nonhistone; HMG4; High mobility group (nonhistone chromosomal) protein 4; High mobility group box 3 ; High mobility group protein 2a; High mobility group protein 4; High mobility group protein B3; High mobility group protein HMG4 ; HMG 4 ; HMG-2a; HMG-4; HMG2A; HMGB 3 ; Hmgb3; HMGB3_HUMAN; MGC90319; Non histone chromosomal protein; Nonhistone chromosomal protein HMG4
Species
Homo sapiens (Human)
Expression Region
1-200
Target Protein Sequence
MAKGDPKKPK GKMSAYAFFV QTCREEHKKK NPEVPVNFAE FSKKCSERWK TMSGKEKSKF DEMAKADKVR YDREMKDYGP AKGGKKKKDP NAPKRPPSGF FLFCSEFRPK IKSTNPGISI GDVAKKLGEM WNNLNDSEKQ PYITKAAKLK EKYEKDVADY KSKGKFDGAK GPAKVARKKV EEEDEEEEEE EEEEEEEEDE
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters. Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor. Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling.
Gene References into Functions
  1. HMGB3 is involved in malignant transformation of hepatocytes and could be a useful biomarker for diagnosis and a potential target for therapy of liver cancer. PMID: 30166860
  2. The study strongly suggests that HMGB3 promotes GBM oncogenesis through the MAPK signalling pathway while miR-200b-3p and miR-200c-3p inhibit its expression. PMID: 30232806
  3. Luciferase reporter assays showed that HMGB3 was directly regulated by miR-205-5p in prostate cancer cells. PMID: 29196733
  4. HMGB3 regulates the genes expression of WNT/beta-catenin pathway PMID: 28678825
  5. Therefore, we infer that HMGB3 is a functional target of miR-27b in modulation of tamoxifen resistance and EMT. PMID: 27363334
  6. these findings demonstrate that HMBG3 may be a potential molecular marker for predicting the prognosis of esophageal squamous cell carcinoma patients PMID: 25755721
  7. our data suggests HMGB3 may serve as an important oncoprotein and indicate that overexpression of HMGB3 in urinary bladder cancer could be used as a potential prognostic marker. PMID: 25647262
  8. High expression of HMGB3 is associated with lymph node metastases in gastric cancer. PMID: 25095979
  9. In this family, microphthalmia, microcephaly, intellectual disability, and short stature are associated with a mutation on the X chromosome in the HMGB3 gene. PMID: 24993872
  10. regulation of HMGB3 by miR-205 reduced both proliferation and invasion of breast cancer cells. PMID: 24098490
  11. Association between co-expression networks involving HMGB3 and differentially-expressed genes in gastric cancer. PMID: 24338397
  12. HMGB3 has a role in gastric cancer disease onset and progression by regulating the cell cycle PMID: 23326140
  13. HMGB3 plays an important role in non-small cell lung cancer progression. PMID: 23609034
  14. Data indicate that myomiR-206 modulates Hmgb3 expression during myogenesis. PMID: 22912879

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Involvement in disease
Microphthalmia, syndromic, 13 (MCOPS13)
Subcellular Location
Nucleus. Chromosome. Cytoplasm.
Protein Families
HMGB family
Tissue Specificity
Expressed predominantly in placenta.
Database Links

HGNC: 5004

OMIM: 300193

KEGG: hsa:3149

STRING: 9606.ENSP00000359393

UniGene: Hs.19114

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