MED27 Antibody

Datasheet

Code	CSB-PA764716LA01HU
Size	US$166
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Image	Western blot All lanes: MED27 antibody at 0.5µg/ml + A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 36, 32, 15 kDa Observed band size: 36 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MED27 Polyclonal antibody

Uniprot No.

Q6P2C8

Target Names

MED27

Alternative Names

Cofactor required for Sp1 transcriptional activation subunit 8 (34kD) antibody; Cofactor required for Sp1 transcriptional activation subunit 8 34kDa antibody; Cofactor required for Sp1 transcriptional activation subunit 8 antibody; CRAP 34 antibody; CRAP34 antibody; CRSP 34 antibody; CRSP 8 antibody; CRSP complex subunit 8 antibody; CRSP34 antibody; CRSP8 antibody; MED 27 antibody; Med27 antibody; MED27_HUMAN antibody; Mediator complex subunit 27 antibody; Mediator of RNA polymerase II transcription subunit 27 antibody; MGC11274 antibody; P37 TRAP/SMCC/PC2 subunit antibody; Transcriptional coactivator CRSP34 antibody; TRAP 37 antibody; TRAP37 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Mediator of RNA polymerase II transcription subunit 27 protein (39-108AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MED27 Antibody (Product code: CSB-PA764716LA01HU) is Non-conjugated. For MED27 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA764716LB01HU	MED27 Antibody, HRP conjugated	ELISA
FITC	CSB-PA764716LC01HU	MED27 Antibody, FITC conjugated
Biotin	CSB-PA764716LD01HU	MED27 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Gene References into Functions

The findings of the present study may aid in the clarification of the function of miR18a, particularly as regards its role in the regulation of Osteosarcoma (OS)cell apoptosis, and indicate that MED27 may be a potential novel therapeutic target in the treatment of OS. PMID: 29693135
MED27 promotes melanoma growth by targeting AKT/MAPK and NF-kappaB/iNOS signaling pathways. PMID: 26797421

Subcellular Location

Nucleus.

Protein Families

Mediator complex subunit 27 family

Database Links

HGNC: 2377

OMIM: 605044

KEGG: hsa:9442

STRING: 9606.ENSP00000292035

UniGene: Hs.374262

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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