NOVA1 Antibody

Datasheet

Code	CSB-PA015957LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Jurkat whole cell lysate All lanes: NOVA1 antibody at 2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 20, 50, 52 kDa Observed band size: 53, 50 kDa IHC image of CSB-PA015957LA01HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NOVA1 Polyclonal antibody

Uniprot No.

P51513

Target Names

NOVA1

Alternative Names

Neuro oncological ventral antigen 1 antibody; Neuro-oncological ventral antigen 1 antibody; NOVA 1 antibody; Nova-1 antibody; Nova1 antibody; NOVA1_HUMAN antibody; Onconeural ventral antigen 1 antibody; Paraneoplastic Ri antigen antibody; RNA binding protein Nova 1 antibody; RNA-binding protein Nova-1 antibody; Ventral neuron specific protein 1 antibody; Ventral neuron-specific protein 1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human RNA-binding protein Nova-1 protein (6-174AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NOVA1 Antibody (Product code: CSB-PA015957LA01HU) is Non-conjugated. For NOVA1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA015957LB01HU	NOVA1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA015957LC01HU	NOVA1 Antibody, FITC conjugated
Biotin	CSB-PA015957LD01HU	NOVA1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Functions to regulate alternative splicing in neurons by binding pre-mRNA in a sequence-specific manner to activate exon inclusion or exclusion. It binds specifically to the sequences 5'-YCAY-3' and regulates splicing in only a subset of regulated exons. Binding to an exonic 5'-YCAY-3' cluster changes the protein complexes assembled on pre-mRNA, blocking U1 snRNP binding and exon inclusion, whereas binding to an intronic 5'-YCAY-3' cluster enhances spliceosome assembly and exon inclusion. Binding to 5'-YCAY-3' clusters results in a local and asymmetric action to regulate spliceosome assembly and alternative splicing in neurons. Binding to an exonic 5'-YCAY-3' cluster changed the protein complexes assembled on pre-mRNA, blocking U1 snRNP (small nuclear ribonucleoprotein) binding and exon inclusion, whereas binding to an intronic 5'-YCAY-3' cluster enhanced spliceosome assembly and exon inclusion. With NOVA1, they perform unique biological functions in different brain areas and cell types. Autoregulates its own expression by acting as a splicing repressor. Acts to activate the inclusion of exon E3A in the glycine receptor alpha-2 chain and of exon E9 in gamma-aminobutyric-acid receptor gamma-2 subunit via a distal downstream UCAU-rich intronic splicing enhancer. Acts to regulate a novel glycine receptor alpha-2 chain splice variant (alpha-2N) in developing spinal cord.

Gene References into Functions

It regulates the alternative splicing of estrogen receptor beta in the aging brain. PMID: 29031089
overexpression of miR-203a-3p leads to a decrease of NOVA1, counter-balanced by an increase of IKAP, supporting a potential interaction between NOVA1 and IKAP. PMID: 27483351
NOVA1 suppression was frequently noted in the gastric cancer microenvironment, and attenuated NOVA1 expression in tumor cells was associated with tumor progression and poor prognosis. PMID: 27318497
Nova1 interacts with GABAARgamma2 not only in the central nervous system but also in hepatocellular carcinoma. Nova1's potential mechanism as an oncogene may due to its interaction with GABAA Rgamma2. PMID: 27733149
Loss of NOVA1 is associated with gastric cancer. PMID: 26673617
Quantitative proteomic analysis was performed to help elucidate a molecular distinction between glioblastoma and oligodendroglioma; analysis showed HSPB1 and NOVA1 to be discriminating factors. PMID: 26108672
Data indicate RNA binding protein NOVA1 as a target of microRNA miR-339. PMID: 26391641
MiR-181b-5p is a tumor suppressor in astrocytoma that inhibits tumor progression by targeting NOVA1. PMID: 25299073
High expression of NOVA1 correlates with poor prognosis in hepatocellular carcinoma. PMID: 24608171
a regulation of LEDGF interaction with chromatin by cellular partners of its PWWP domain could be involved in several processes linked to LEDGF tethering properties, such as lentiviral integration, DNA repair or transcriptional regulation PMID: 24312278
Gene silencing and overexpression of the nELAV member HuD in motoneuronal NSC34 cells indicate that Nova1 mRNA stability and translation are positively and strongly controlled by the nELAV proteins PMID: 18218628
Semiquantitative estimation of circulating anti-Ri antibodies demonstrated a renewed increase in antibody levels preceding relapse of the cancer, as confirmed using F-fluorodeoxyglucose positron emission tomography (FDG-PET) PMID: 18822086

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Subcellular Location

Nucleus.

Tissue Specificity

Expressed in cerebellum, brain stem, hippocampus, and frontal cortex.

Database Links

HGNC: 7886

OMIM: 602157

KEGG: hsa:4857

STRING: 9606.ENSP00000438875

UniGene: Hs.31588

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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