PPP1R8 Antibody

Datasheet

Code	CSB-PA618641DSR1HU
Size	US$166
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Image	Western blot All lanes: PPP1R8 antibody at 4.73µg/ml Lane 1: PC-3 whole cell lysate Lane 2: HL60 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 39, 23, 14 kDa Observed band size: 39 kDa Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA618641DSR1HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA618641DSR1HU at dilution of 1:100 Immunofluorescent analysis of HepG2 cells using CSB-PA618641DSR1HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PPP1R8 Polyclonal antibody

Uniprot No.

Q12972

Target Names

PPP1R8

Alternative Names

Activator of RNA decay antibody; ARD 1 antibody; ARD-1 antibody; ARD1 antibody; Homolog of E.coli RNase E antibody; NIPP 1 antibody; NIPP-1 antibody; NIPP1 antibody; Nuclear inhibitor of protein phosphatase 1 antibody; nuclear inhibitor of protein phosphatase-1 alpha antibody; Nuclear subunit of PP1 antibody; PP1R8_HUMAN antibody; PPP1R8 antibody; PRO2047 antibody; Protein phosphatase 1 regulatory inhibitor subunit 8 antibody; Protein phosphatase 1 regulatory subunit 8 antibody; RNase E antibody; RNase E, E. coli, homolog of antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Nuclear inhibitor of protein phosphatase 1 protein (1-209AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.; Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.

Gene References into Functions

NIPP1 could be activated by hypoxia and contributed to hypoxia-induced invasive and metastatic potential in hepatocellular carcinoma. PMID: 27644248
Data suggest overexpression of NIPP1 in HeLa cells causes induction of genes of mesenchymal lineage with reduction in cell proliferation; thus, key dephosphorylation events may be involved in epithelial-mesenchymal transition or transdifferentiation. PMID: 25907536
The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assembly PMID: 11909864
NIPP1 has a role in the nuclear targeting and/or retention of PP1 PMID: 15501817
Required for the global trimethylation of histone3 at lysine27 and is implicated in gene silencing by enhancer of zeste homolog (EZH)2. PMID: 17724462
NIPP1 is present in a complex with EED and EZH2 in vivo and has distinct binding sites for these proteins. PMID: 17804093
NMR structure of the NIPP1 FHA domain. PMID: 18253837
NIPP1 works as a molecular sensor for PP1 to recognize phosphorylated Sap155. PMID: 18842582

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Subcellular Location

Nucleus. Nucleus speckle. Note=Primarily, but not exclusively, nuclear.; [Isoform Gamma]: Cytoplasm. Note=Found mainly in the cytoplasm.

Tissue Specificity

Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform

Database Links

HGNC: 9296

OMIM: 602636

KEGG: hsa:5511

STRING: 9606.ENSP00000311677

UniGene: Hs.533474

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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