Phospho-AKT1/AKT2/AKT3 (Tyr315/316/312) Antibody

Code CSB-PA569237
Size US$360
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  • Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using AKT1/AKT2/AKT3(phospho-Tyr315/316/312) Antibody.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3(Phospho-Tyr315/316/312) Antibody(left) or the same antibody preincubated with blocking peptide(right).
  • Immunofluorescence staining of methanol-fixed Hela cells using AKT1/AKT2/AKT3(phospho-Tyr315/316/312) Antibody.
  • Western blot analysis of extracts from 3T3 cells, treated with EGF or calf intestinal phosphatase (CIP), using AKT1/AKT2/AKT3 (phospho-Tyr315/316/312) Antibody.
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) AKT1/AKT2/AKT3 Polyclonal antibody
Uniprot No.
Target Names
AKT1/AKT2/AKT3
Raised in
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 315/316/312 (P-E-Y(p)-L-A) derived from Human AKT1/AKT2/AKT3.
Immunogen Species
Homo sapiens (Human)
Clonality
Polyclonal
Purification Method
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Concentration
It differs from different batches. Please contact us to confirm it.
Form
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications
ELISA,WB,IHC,IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:1000
IHC 1:50-1:100
IF 1:100-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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