RNF144B Antibody

Code CSB-PA003683
Size US$100
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  • Western Blot analysis of HeLa cells using p53RFP Polyclonal Antibody
  • Western Blot analysis of RAT-MUSCLE HELA 293T AD293 cells using p53RFP Polyclonal Antibody
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Product Details

Uniprot No.
Target Names
RNF144B
Alternative Names
RNF144B; IBRDC2; P53RFP; E3 ubiquitin-protein ligase RNF144B; IBR domain-containing protein 2; RING finger protein 144B; p53-inducible RING finger protein
Raised in
Rabbit
Species Reactivity
Human,Mouse
Immunogen
Synthesized peptide derived from the Internal region of Human p53RFP.
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Isotype
IgG
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form
Liquid
Tested Applications
WB, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:40000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.
Gene References into Functions
  1. Low RNF144B expression is associated with gastric cancer. PMID: 27524417
  2. findings substantiate PIR2/Rnf144b as a potentially critical component of epithelial homeostasis, acting downstream of DeltaNp63alpha to regulate cellular levels of p21(WAF1/CIP1) and DeltaNp63alpha PMID: 23128396
  3. PIR2, by being induced by TAp73 and degrading DeltaNp73, differentially regulates TAp73/DeltaNp73 stability. PMID: 20615966
  4. PIR2/RNF144B/IBRDC2 regulates the stability of TAp73 and DNp73. PMID: 20615966
  5. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
  6. IBRDC2, an IBR-type RING-finger E3 ubiquitin ligase, regulates the levels of Bax and protects cells from unprompted Bax. activation and cell death. PMID: 20300062
  7. p53RFP, a p53-inducible RING-finger protein, regulates the stability of p21WAF1. PMID: 12853982

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Subcellular Location
Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation.
Protein Families
RBR family, RNF144 subfamily
Tissue Specificity
Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis.
Database Links

HGNC: 21578

KEGG: hsa:255488

STRING: 9606.ENSP00000259939

UniGene: Hs.148741

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