RPL19 Antibody

Datasheet

Code	CSB-PA020160LA01HU
Size	$600
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Image	Western Blot Positive WB detected in: Hela whole cell lysate(20µg), MCF7 whole cell lysate(20µg), PC-3 whole cell lysate(20µg), HEK293 whole cell lysate(20µg), HepG2 whole cell lysate(20µg) All lanes: RPL19 antibody at 1:500 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 23,28 kDa Observed band size: 28 kDa Exposure time: 180s IHC image of CSB-PA020160LA01HU diluted at 1:50 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HepG2 cell with CSB-PA020160LA01HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). IHC image of CSB-PA020160LA01HU diluted at 1:50 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RPL19 Polyclonal antibody

Uniprot No.

P84098

Target Names

RPL19

Alternative Names

60S ribosomal protein L19 antibody; DKFZp779D216 antibody; FLJ27452 antibody; HGNC:10312 antibody; L19 antibody; MGC71997 antibody; Ribosomal Protein L19 antibody; Ribosomal protein L19; cytosolic; N terminus truncated antibody; RL19_HUMAN antibody; rpl19 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human 60S ribosomal protein L19 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The RPL19 Antibody (Product code: CSB-PA020160LA01HU) is Non-conjugated. For RPL19 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA020160LB01HU	RPL19 Antibody, HRP conjugated	ELISA
FITC	CSB-PA020160LC01HU	RPL19 Antibody, FITC conjugated
Biotin	CSB-PA020160LD01HU	RPL19 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200
IF	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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