RPN2 Antibody

Datasheet

Code	CSB-PA020345ESR2HU
Size	US$166
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Image	Western blot All lanes: RPN2 antibody at 1.91µg/ml Lane 1: NIH/3T3 whole cell lysate Lane 2: 293T whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Human placenta tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 70, 68 kDa Observed band size: 70 kDa Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA020345ESR2HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA020345ESR2HU at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RPN2 Polyclonal antibody

Uniprot No.

P04844

Target Names

RPN2

Alternative Names

RPN2; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit; RIBIIR; Ribophorin II; RPN-II; Ribophorin-2

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 protein (360-540AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

Gene References into Functions

Rpn13-Rpn2 complex structural analysis shows that RA190 targets hRpn13 and Uch37 through parallel mechanisms and at proteasomes, RA190-inactivated Uch37 cannot disassemble hRpn13-bound ubiquitin chains PMID: 28598414
These findings indicated a novel pathway of tumor-promoting activity by RPN2 in colorectal cancer (CRC), with significant implications for unraveling the tumorigenesis of CRC. PMID: 29749494
RPN2 expression correlates with gastric adenocarcinoma cell invasion and shows promise as a new prognostic factor in human gastric adenocarcinoma PMID: 28035352
Data suggest that RPN2 is involved in the regulation of lethal cancer phenotypes and represents a promising new target for RNAi-based medicine against non-small cell lung cancer. PMID: 25595901
Of the total, the deregulation of several genes (CDK1, CDK2, CDK4, MCM2, MCM3, MCM4, EIF3a and RPN2) were potentially associated with disease development and progression. PMID: 24386425
these results indicated that high glycosylation of CD63 by RPN2 is implicated in clinical outcomes in breast cancer patients PMID: 24884960
Furthermore, our study reveals that high expression of RPN2 and concomitant accumulation of mtp53 were associated with cancer tissues in a small cohort of metastatic breast cancer patients. PMID: 23959174
Rpn13 binding to the proteasome scaffolding protein hRpn2/S1 abrogates its interdomain interactions, thus activating hRpn13 for ubiquitin binding. PMID: 20471946
RPN2 gene confers docetaxel resistance in breast cancer. PMID: 18724378
S-glutathionylation of Rpn2 is a contributory mechanism for H2O2-induced inhibition of 26 S proteasomal function PMID: 19549781
Data show that the decay of metabolically labeled p53 follows biphasic kinetics, while 20 S proteasome executes the first phase by default, and the second phase requires the 26 S proteasome. PMID: 19617345

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Subcellular Location

Endoplasmic reticulum. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

SWP1 family

Tissue Specificity

Expressed in all tissues tested.

Database Links

HGNC: 10382

OMIM: 180490

KEGG: hsa:6185

STRING: 9606.ENSP00000237530

UniGene: Hs.370895

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Related pathways

Protein processing in endoplasmic reticulum

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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