Code | CSB-PA336163EA01HIY |
Size | US$166 |
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The S Antibody (Product code: CSB-PA336163EA01HIY) is Non-conjugated. For S Antibody with conjugates, please check the following table.
Rabbits were immunized with recombinant human coronavirus OC43 spike glycoprotein protein (15-344aa) to generate the CSB-PA321224LA01BUA polyclonal antibody against the spike protein. The spike protein of human coronavirus OC43 is an essential component of the virus and plays a crucial role in its pathogenesis, making it a potential target for the development of vaccines and therapeutics.
Following protein G purification, the rabbit anti-human coronavirus OC43 S polyclonal antibody achieved a purity level of 95%. This non-conjugated IgG specifically binds to the spike protein of human coronavirus OC43 and can detect endogenous levels of the protein, as demonstrated by ELISA.
Applications : Western Blot Assay
Sample type: Cells
Review: Western blot of the lysates of HCoV-OC43-infected MRC-5 cell streated with kurarinone or vehicle and evaluated at 1,2,3, and 4dpi. The HCoV-OC43 Spike protein was detected and indicated by an arrow head as shown; β-actin was used as a loading control.
By Anonymous
Applications : Immunofluorescence experiments
Sample type: cells
Review: To assess whether 5 min of various UVC illumination reduces the number of infected cells, immunostaining was performed to detect the presence of OC43 viral particles in the host human cells.
By Anonymous
Applications : Western Blot Analysis
Sample type: cell
Review: In MRC-5 and A549 cells was detected using Western blot analysis following HCoV-OC43 infection and vehicle or 10 μM CDN treatment at 1–4 dpi. β-actin was used as the internal control.
By Anonymous