SDHA Antibody

Code CSB-PA01989A0Rb
Size US$166
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  • Western blot
    All lanes: SDHA antibody at 10µg/ml
    Lane 1: Rat heart tissue
    Lane 2: Mouse spleen tissue
    Lane 3: Mouse liver tissue
    Lane 4: Hela whole cell lysate
    Lane 5: Zebrafish lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 73, 68, 57 kDa
    Observed band size: 73 kDa

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA01989A0Rb at dilution of 1:50

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) SDHA Polyclonal antibody
Uniprot No.
Target Names
SDHA
Alternative Names
CMD1GG antibody; DHSA_HUMAN antibody; Flavoprotein subunit of complex II antibody; Fp antibody; PGL5 antibody; SDH 2 antibody; SDH1 antibody; SDH2 antibody; SDHA antibody; SDHF antibody; Succinate dehydrogenase [ubiquinone] flavoprotein subunit antibody; Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial antibody; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial antibody; Succinate dehydrogenase complex flavoprotein subunit A antibody; Succinate dehydrogenase complex flavoprotein subunit antibody; Succinate dehydrogenase complex flavoprotein subunit precursor antibody; Succinate dehydrogenase complex subunit A antibody; Succinate dehydrogenase complex subunit A flavoprotein (Fp) antibody; Succinate dehydrogenase complex subunit A flavoprotein antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse, Rat, Zebrafish
Immunogen
Recombinant Human Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial protein (44-664AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The SDHA Antibody (Product code: CSB-PA01989A0Rb) is Non-conjugated. For SDHA Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA01989B0Rb SDHA Antibody, HRP conjugated ELISA
FITC CSB-PA01989C0Rb SDHA Antibody, FITC conjugated
Biotin CSB-PA01989D0Rb SDHA Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function
Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.
Gene References into Functions
  1. The six index cases described here presented with a variety of clinical manifestations extending the known phenotypic spectrum in SDHA disease. PMID: 28500238
  2. Report left ventricular non-compaction associated with Barth Syndrome due to triple mutations in TAZ, DTNA, and SDHA genes in multiple members of one family. PMID: 29508483
  3. We used a yeast model to characterize 22 SDHA VUS. These data revealed 16 (73%) of SDHA VUS as loss of function (and therefore pathogenic), highlighting the importance of understanding such variants to provide better clinical recommendations for genetic counselors concerning family screening and early detection protocols. PMID: 28724664
  4. Germline SDHA mutations are relatively common (7.6%) in patients with genetically unexplained paraganglioma (PGL). Most index patients presented with apparently sporadic PGL. In this SDHA series, the largest assembled so far, we found the lowest penetrance of all major PGL predisposition genes. PMID: 29177515
  5. We found that microRNA 31 (miR-31) suppressed succinate dehydrogenase complex subunit A (SDHA) expression, vital for mitochondrial electron transport chain (ETC) complex II PMID: 27346679
  6. This is only the second report supporting the dominant nature of the SDHA c.1351C>T (p.Arg451Cys) mutation being causative for an autosomal dominantly inherited mitochondrial metabolic disorder expanding the phenotypic presentation to an earlier onset of disease with additional cardiac involvement. PMID: 27683074
  7. The SDHA, TMEM127, MAX, and SDHAF2 genes contribute to hereditary pheochromocytoma and paraganglioma. PMID: 28384794
  8. SDH-deficient gastrointestinal stromal tumors (GISTs) account for approximately 8% of gastric GISTs and are associated with a high rate of distant metastasis, regardless of conventional risk category. PMID: 27340750
  9. For classification, tumors were characterized by SDHA, B, C, or D (SDHX) mutations and other genetic and epigenetic alterations, including presence of mutations in germline PMID: 27011036
  10. data show that SDHA flavination is independent of SDHAF2 in breast cancer cells, employing an alternative mechanism. PMID: 27587393
  11. FAD interacts noncovalently with SDHA in the absence of SDH5 PMID: 27296776
  12. After indirect co-culture, OP was increased in the BxPc-3 and Panc-1 cells; correspondingly, succinate dehydrogenase, FH and MCT expression were increased. After the MCT1-specific inhibitor removed 'tumor-stromal' metabolic coupling, the migration and invasion abilities of the pancreatic cancer cells were decreased. PMID: 28260082
  13. Data suggest that succinate dehydrogenases SDHA and SDHB immunohistochemistry should be interpreted with caution, due to possible false-positive or false-negative results, and ideally in the setting of quality assurance provided by molecular testing. PMID: 28179334
  14. Combined blockade of CDK and SDH, both genetically and pharmaceutically, showed synergy and resulted in inhibited proliferation, migration, invasion and migration in A2780 cells Cyclin E-driven OvCa cells appeared addicted to glucose metabolism via TCA. Combined CDKi with modalities targeting TCA, like SDHA inhibition showed promising effects for this genotype. PMID: 26826064
  15. According to international guidelines, SDHB, SDHC, and SDHD genetic testing were performed in this patient, but not SDHA, which would have been prescribed only after surgery, in case of SDHA negative immunohistochemistry PMID: 26490314
  16. As a similar defect of succinate dehydrogenase is apparent in patient cell-derived cardiomyocytes, the authors conclude that these defects represent a molecular basis for the cardiac pathology in Barth syndrome. PMID: 26697888
  17. our findings provide further evidence that patients with KIT/PDGFRA wild-type SDH-deficient GIST harboring SDHA mutations experience good survival outcomes PMID: 25188872
  18. This study strengthens the etiological association of SDH genes with pituitary neoplasia, renal tumorigenesis, and gastric gastrointestinal stromal tumors. Also, pancreatic neuroendocrine tumor falls within the SDH-related tumor spectrum. PMID: 26259135
  19. Three novel mutations in SDHA were found in patients presenting Leigh syndrome (LS) and/or leukodystrophy. PMID: 24781757
  20. Overall, 9 of the 34 patients with KIT/PDGFRA wild-type GIST carried mutations in one of the four subunits of the SDH complex (six patients in SDHA, two in SDHB, one in SDHC PMID: 23612575
  21. SDH deficiency may promote tumorigenesis through accumulation of succinate and inhibition of dioxygenase enzymes. Inhibition of TET activity may, in turn, alter global DNA methylation and gene expression in SDH-deficient tumors. PMID: 23743927
  22. Data indicate that SDH5 is protected from mitochondrial LON protease (LONM)-mediated degradation in mitochondria by its stable interaction with SDHA, a state that is dysregulated in hereditary paraganglioma 2 (PGL2). PMID: 24414418
  23. Electron transport complex-II and manganese superoxide dismutase (MnSOD) enzyme activities were decreased in obese compared with non-obese pregnant women. PMID: 23956348
  24. A significant subset of bladder paragangliomas is SDH deficient PMID: 23797725
  25. SDHA immunohistochemistry on gastrointestinal stromal tumors can identify the presence of an SDHA germline mutation. PMID: 23174939
  26. In the paraganglioma of the proband, in addition to the germline mutation, a somatic mutation was observed (c.1865G>A, p.Trp622*). PMID: 23633203
  27. Studies indicate that mutations in the mitochondrial complex II structural subunit genes SDHB, SDHC and SDHD and the regulatory subunit gene SDHAF2 in many paraganglioma families. PMID: 23291190
  28. Loss of SDHA expression in gastrointestinal stromal tumor (GIST) reliably predicts the presence of SDHA mutations, which represent a relatively common cause of SDH-deficient GIST in adults. PMID: 22955521
  29. Studies indicate that the pH change leads to the dissociation of SDHA and SDHB subunits from the remaining membrane-anchored subunits and the consequent block of enzymatic succinate-ubiquinone reductase (SQR) activity. PMID: 23000077
  30. Studies indicate that an array of tumor syndromes caused by complex II-associated mutations in genes SDHA, SDHB, SDHC, SDHD, SDHAF1 and SDHAF2 have been identified over a decade. PMID: 23174333
  31. Studies indicate that the flavinylation factor Sdh5 (SDHAF2) provided insight into the possible mechanism associated with Sdh1 (SDHA) flavinylation. PMID: 23380393
  32. A germline p.Arg31X nonsense SDHA mutation was identified in one of the six wild-type gastrointestinal stromal tumors cases. An additional SDHA missense mutation was identified in the extended KIT/PDGFRA WT GIST patients cohort. PMID: 22974104
  33. Data indicate that SDHB-deficiency was tightly associated with overexpression of IGF1R protein and transcript, and Biallelic inactivation of the SDHA gene was identified in 5 of 11 SDHB-negative gastrointestinal stromal tumors. PMID: 23109135
  34. This report represents the first example of SDHB mutation as a cause of inherited mitochondrial respiratory chain disease and extends the SDHA mutation spectrum in patients with isolated complex II deficiency. PMID: 22972948
  35. Loss of SDHA expression identifies SDHA mutations in succinate dehydrogenase-deficient gastrointestinal stromal tumors. PMID: 23060355
  36. SDHA-negative gastrointestinal stromal tumors comprise approximately 30% of SDHB-negative/SDH-deficient gastrointestinal stromal tumors, and SDHA loss generally correlates with SDHA mutations. PMID: 23282968
  37. Tumor-derived FH and SDH mutations accumulate fumarate and succinate, leading to enzymatic inhibition of multiple alpha-KG-dependent dioxygenases and consequent alterations of genome-wide histone and DNA methylation. PMID: 22677546
  38. First report describing germline and somatic loss-of-function mutations in SDHA that are linked to the development of sporadic KIT/PDGFRA wild-type GISTs. PMID: 21505157
  39. Cells with Complex II defect may undergo a progressive mitochondrial dysfunction, characterized by Dcmit loss, Calcium overload and increased ROS, eventually leading to cell death. PMID: 20489732
  40. study presents the association of a mutation in the SDHA gene with recessive neonatal isolated dilated cardiomyopathy in 15 patients of two large consanguineous Bedouin families PMID: 20551992
  41. Decreased electron Transport Complex II activity is associated with ulcerative colitis. PMID: 20440543
  42. Mutations in electron Transport Complex II is associated with Leber hereditary optic neuropathy failing to compensate for impaired oxidative phosphorylation. PMID: 19836344
  43. Review. Succinate dehydrogenase catalyses a step in the Krebs tricarboxylic-acid cycle. Inherited heterozygous mutations in the gene encoding this enzyme causes a predisposition to inherited neoplasia syndromes. PMID: 12612654
  44. The SDHA variants that have increased in frequency during human evolution might, by influencing the regulation of cellular oxygen homeostasis, confer protection against certain environmental toxins or pathogens that are prevalent in Africa. PMID: 17376234
  45. Phosphorylation of flavoprotein subunit ofsuccinate-ubiquinone reductase might be important for maintaining mitochondrial energy metabolism within the tumor microenvironment. PMID: 19644226

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Involvement in disease
Mitochondrial complex II deficiency (MT-C2D); Leigh syndrome (LS); Cardiomyopathy, dilated 1GG (CMD1GG); Paragangliomas 5 (PGL5)
Subcellular Location
Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Protein Families
FAD-dependent oxidoreductase 2 family, FRD/SDH subfamily
Database Links

HGNC: 10680

OMIM: 252011

KEGG: hsa:6389

STRING: 9606.ENSP00000264932

UniGene: Hs.440475

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