SPOCK1 Antibody

Datasheet

Code	CSB-PA730785LA01HU
Size	US$166
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Image	Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA730785LA01HU at dilution of 1:100 Immunofluorescent analysis of HepG2 cells using CSB-PA730785LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) Western Blot Positive WB detected in: Mouse brain tissue All lanes: SPOCK1 antibody at 1:3000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa Western Blot Positive WB detected in: 293T whole cell lysate All lanes: SPOCK1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SPOCK1 Polyclonal antibody

Uniprot No.

Q08629

Target Names

SPOCK1

Alternative Names

SPOCK1 antibody; SPOCK antibody; TIC1 antibody; TICN1 antibody; Testican-1 antibody; Protein SPOCK antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Testican-1 protein (290-435AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SPOCK1 Antibody (Product code: CSB-PA730785LA01HU) is Non-conjugated. For SPOCK1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA730785LB01HU	SPOCK1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA730785LC01HU	SPOCK1 Antibody, FITC conjugated
Biotin	CSB-PA730785LD01HU	SPOCK1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:2000-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The SPOCK1 polyclonal antibody CSB-PA730785LA01HU was produced in the rabbit immunized with the recombinant human Testican-1 protein (290-435aa). The target protein SPOCK1, also known as Testican-1 protein, mainly modulates cell adhesion, migration, and proliferation by interacting with other ECM proteins, proteases, and growth factors.

This SPOCK1 antibody was tested in the ELISA, WB, IHC, and IF applications. The non-conjugated IgG got purified by protein G affinity chromatography and reached up to 95% in purity. It reacts with the SPOCK1 proteins from human and mouse samples.

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Target Background

Function

May play a role in cell-cell and cell-matrix interactions. May contribute to various neuronal mechanisms in the central nervous system.

Gene References into Functions

Testican-1 blood level is related to the severity of sepsis and Testican-1 could be used as a biomarker to determine the severity of sepsis. PMID: 29315764
Overexpression of SPOCK1was confirmed in head and neck squamous cell carcinoma clinical specimens. Overexpression of SPOCK1 contributes to the aggressive nature of HNSCC. PMID: 29233721
High SPOCK1 expression is associated with castration-resistant prostate cancer. PMID: 28534948
downregulation of both strands of pre-miR-150 and overexpression of SPOCK1 are involved in esophageal squamous cell carcinoma (ESCC). pathogenesis. The involvement of passenger strand miRNAs in the regulation of cancer cell aggressiveness is a novel concept in RNA research PMID: 28659612
is significantly upregulated in osteosarcoma tissue. PMID: 28869894
Functional assessment in cocultures demonstrated that SPOCK1 strongly affects the composition of the extracellular collagen matrix and by doing so, enables invasive tumor cell growth in Pancreatic ductal adenocarcinoma. PMID: 28486750
Transcriptomic analysis of EPCR-silenced tumors unveiled an effect mediated by matricellular SPOCK1/testican 1. SPOCK1 silencing suppressed in vitro 3D growth. SPOCK1 ablation decreased orthotopic tumor growth and reduced metastatic osteolytic tumors. High SPOCK1 levels were also associated with poor clinical outcome in a subset of breast cancer patients. PMID: 28103946
a novel TGF-beta-induced myoepithelial marker and enhanced invasion in breast cancer cells and correlated with poor prognosis PMID: 27626636
Our results suggested that microRNA-129-5p could directly specifically suppress SPOCK1, which might be one of the potential mechanisms in inhibiting cell processes including viability, proliferation, cell mitosis, migration, and invasiveness of gastric cancer cells. PMID: 28653880
SPOCK1 expression is significantly up-regulated in colorectal cancer tissues and associated with tumor size and stage. SPOCK1 stimulates the PI3K/AKT signaling pathway to inhibit cell apoptosis and promote tumor growth. PMID: 27889608
findings suggest that SPOCK1 is a critical mediator of tumor growth and metastasis in prostate cancer PMID: 27486308
Knockdown of SPOCK1 inhibits the proliferation. PMID: 28281964
This study suggests that SPOCK1 promotes proliferation, migration and invasion in glioma cells by activating PI3K/AKT and Wnt/beta-catenin pathways, which provides a potential theoretical basis for clinical treatment of glioma. PMID: 27108836
SPOCK1 is highly expressed in ovarian cancer.SPOCK1 contributes to ovarian cancer development and metastasis. PMID: 26638890
These results suggest that up-regulation of SPOCK1 in ESCC induces EMT, thus promotes migration and invasion in ESCC cells. PMID: 26077618
Collectively, altered expression of PKP1 and SPOCK1 appears to be a frequent and critical event in prostate cancer PMID: 26138584
SPOCK1 activates PI3K/Akt signaling. PMID: 25623055
Testican-1-induced EMT signaling. PMID: 23873022
this study showed that SPOCK1 is a novel metastasis related biomarker in lung cancer and may be new diagnostic and therapeutic target for lung cancer. PMID: 24134845
In the present study, we showed that SPOCK1 could inhibit apoptosis and promote cancer invasion. PMID: 23022495
Significant overexpression of osteonectin mRNA is associated with pancreatic cancer PMID: 16596217
SPOCK gene underlies variation of age at menarche PMID: 19282985

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Subcellular Location

Secreted, extracellular space, extracellular matrix.

Database Links

HGNC: 11251

OMIM: 602264

KEGG: hsa:6695

STRING: 9606.ENSP00000282223

UniGene: Hs.596136

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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