TPK1 Antibody

Datasheet

Code	CSB-PA024103ESR2HU
Size	US$166
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Image	Western Blot Positive WB detected in: U251 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), K562 whole cell lysate(30µg), CT-26 whole cell lysate(30µg), PC-3 whole cell lysate(30µg),JK whole cell lysate(30µg),Mouse Liver tissue lysate(30µg),Mouse Kidneyr tissue lysate(30µg) All lanes: TPK1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 27,14kDa Observed band size: 27 kDa Exposure time: 180s IHC image of CSB-PA024103ESR2HU diluted at 1:100 and staining in paraffin-embedded human Kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody IHC image of CSB-PA024103ESR2HU diluted at 1:100 and staining in paraffin-embedded human Heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody IHC image of CSB-PA024103ESR2HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TPK1 Polyclonal antibody

Uniprot No.

Q9H3S4

Target Names

TPK1

Alternative Names

hTPK1 antibody; Placental protein 20 antibody; PP20 antibody; Thiamin pyrophosphokinase 1 antibody; Thiamine diphosphokinase antibody; Thiamine kinase antibody; Thiamine pyrophosphokinase 1 antibody; THMD5 antibody; TPK1 antibody; TPK1_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from human TPK1:170-220aa

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.

Gene References into Functions

Mutation analysis of TPK1 uncovered three missense, one splice-site, and one frameshift mutation resulting in decreased TPK protein levels PMID: 22152682
findings indicate the importance of the Sp1 transcription factor cis-element in regulating the human thiamin pyrophosphokinase gene (hTPK1) gene expression PMID: 16262001
Genomic variations in either the fetal or maternal TPK1 gene could contribute to variability of birth weight in normal humans. PMID: 17295612
This is the first report on the primary sequence for mammalian thiamin pyrophosphokinase, a protein that catalyzes the pyrophosphorylation of thiamin with adenosine 5'-triphosphate to form thiamin pyrophosphate. PMID: 10567383

Involvement in disease

Thiamine metabolism dysfunction syndrome 5, episodic encephalopathy type (THMD5)

Protein Families

Thiamine pyrophosphokinase family

Tissue Specificity

Detected in heart, kidney, testis, small intestine and peripheral blood leukocytes, and at very low levels in a variety of tissues.

Database Links

HGNC: 17358

OMIM: 606370

KEGG: hsa:27010

STRING: 9606.ENSP00000353165

UniGene: Hs.660232

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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