Human Thiamin pyrophosphokinase 1(TPK1) ELISA kit

Code CSB-EL024103HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
thiamin pyrophosphokinase 1
Alternative Names
hTPK1 ELISA Kit; Placental protein 20 ELISA Kit; PP20 ELISA Kit; Thiamin pyrophosphokinase 1 ELISA Kit; Thiamine diphosphokinase ELISA Kit; Thiamine kinase ELISA Kit; Thiamine pyrophosphokinase 1 ELISA Kit; THMD5 ELISA Kit; TPK1 ELISA Kit; TPK1_HUMAN ELISA Kit
Abbreviation
TPK1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TPK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %91
Range %86-95
1:2Average %102
Range %97-107
1:4Average %91
Range %85-97
1:8Average %97
Range %91-103
Recovery
The recovery of human TPK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-98
EDTA plasma (n=4)9790-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.329 2.368 2.349 2.243
10001.876 1.857 1.867 1.761
5001.339 1.368 1.354 1.248
2500.843 0.839 0.841 0.735
1250.566 0.599 0.583 0.477
62.50.411 0.423 0.417 0.311
31.250.250 0.259 0.255 0.149
00.105 0.107 0.106
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human TPK1 ELISA Kit was designed for the quantitative measurement of Human TPK1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

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Target Background

Function
(From Uniprot)
Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.
Gene References into Functions
  1. Mutation analysis of TPK1 uncovered three missense, one splice-site, and one frameshift mutation resulting in decreased TPK protein levels PMID: 22152682
  2. findings indicate the importance of the Sp1 transcription factor cis-element in regulating the human thiamin pyrophosphokinase gene (hTPK1) gene expression PMID: 16262001
  3. Genomic variations in either the fetal or maternal TPK1 gene could contribute to variability of birth weight in normal humans. PMID: 17295612
  4. This is the first report on the primary sequence for mammalian thiamin pyrophosphokinase, a protein that catalyzes the pyrophosphorylation of thiamin with adenosine 5'-triphosphate to form thiamin pyrophosphate. PMID: 10567383
Involvement in disease
Thiamine metabolism dysfunction syndrome 5, episodic encephalopathy type (THMD5)
Protein Families
Thiamine pyrophosphokinase family
Tissue Specificity
Detected in heart, kidney, testis, small intestine and peripheral blood leukocytes, and at very low levels in a variety of tissues.
Database Links

HGNC: 17358

OMIM: 606370

KEGG: hsa:27010

STRING: 9606.ENSP00000353165

UniGene: Hs.660232

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