Recombinant Human Thiamin pyrophosphokinase 1 (TPK1)

Code CSB-YP024103HU
MSDS
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Source Yeast
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Code CSB-EP024103HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP024103HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP024103HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TPK1
Uniprot No.
Alternative Names
hTPK1; Placental protein 20; PP20; Thiamin pyrophosphokinase 1; Thiamine diphosphokinase; Thiamine kinase; Thiamine pyrophosphokinase 1; THMD5; TPK1; TPK1_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-243
Target Protein Sequence
MEHAFTPLEP LLSTGNLKYC LVILNQPLDN YFRHLWNKAL LRACADGGAN RLYDITEGER ESFLPEFING DFDSIRPEVR EYYATKGCEL ISTPDQDHTD FTKCLKMLQK KIEEKDLKVD VIVTLGGLAG RFDQIMASVN TLFQATHITP FPIIIIQEES LIYLLQPGKH RLHVDTGMEG DWCGLIPVGQ PCMQVTTTGL KWNLTNDVLA FGTLVSTSNT YDGSGVVTVE TDHPLLWTMA IKS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.
Gene References into Functions
  1. Mutation analysis of TPK1 uncovered three missense, one splice-site, and one frameshift mutation resulting in decreased TPK protein levels PMID: 22152682
  2. findings indicate the importance of the Sp1 transcription factor cis-element in regulating the human thiamin pyrophosphokinase gene (hTPK1) gene expression PMID: 16262001
  3. Genomic variations in either the fetal or maternal TPK1 gene could contribute to variability of birth weight in normal humans. PMID: 17295612
  4. This is the first report on the primary sequence for mammalian thiamin pyrophosphokinase, a protein that catalyzes the pyrophosphorylation of thiamin with adenosine 5'-triphosphate to form thiamin pyrophosphate. PMID: 10567383
Involvement in disease
Thiamine metabolism dysfunction syndrome 5, episodic encephalopathy type (THMD5)
Protein Families
Thiamine pyrophosphokinase family
Tissue Specificity
Detected in heart, kidney, testis, small intestine and peripheral blood leukocytes, and at very low levels in a variety of tissues.
Database Links

HGNC: 17358

OMIM: 606370

KEGG: hsa:27010

STRING: 9606.ENSP00000353165

UniGene: Hs.660232

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