Recombinant Mouse C-C motif chemokine 12 protein (Ccl12) (Active)

CCL12 (MCP-5) is a potent monocyte chemoattractant unique to mice, making it an essential tool for studying monocyte recruitment in murine models of inflammation, fibrosis, and tumor biology. This tag-free recombinant protein encompasses the full-length mature sequence (residues 23–104) and demonstrates confirmed biological activity in a chemotaxis bioassay using human peripheral blood monocytes at 10–50 ng/ml, supporting its use in Transwell migration assays, CCR2 signaling pathway studies involving MAPK and NF-κB activation, and as a reference standard in cytokine detection platforms. Endotoxin levels below 1.0 EU/μg minimize the risk of LPS-driven artifacts in sensitive immune cell functional assays, while purity exceeding 95% by SDS-PAGE provides a suitable basis for antibody development workflows including immunization, ELISA coating, and Western blot positive controls. The E. coli-derived protein offers a non-glycosylated format that aligns with standards expected in quantitative binding and activity benchmarking experiments.