Recombinant Rat Cxcl17 protein (Cxcl17) (Active)

CXCL17, a mucosal chemokine implicated in dendritic cell and macrophage recruitment, plays a key role in innate immune surveillance, making functional-grade recombinant protein essential for dissecting its signaling biology. This tag-free rat Cxcl17 encompasses the full-length mature sequence (residues 23–119), produced in *E. coli*, and demonstrates confirmed signaling potency with an ED50 below 5.0 μg/ml in a murine endothelial cell VEGF-induction assay, corresponding to a specific activity exceeding 200 IU/mg — providing a reliable basis for chemotaxis and migration assays, angiogenesis-related signaling studies, and functional screening of downstream pathways including MAPK and NF-κB. Endotoxin levels held below 1.0 EU/μg minimize LPS-driven artifacts, which satisfies the stringent criteria typical for primary immune cell activation assays and *in vivo* rat inflammation models. Purity exceeding 97% by SDS-PAGE further supports use as a reference standard in cytokine detection assays or as a coating antigen for antibody development and ELISA validation.