Product Details

Purity

>96% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 0.1-1.0 μg/ml.

Target Names

Ccl11

Uniprot No.

P97545

Research Area

Immunology

Alternative Names

Ccl11; Scya11Eotaxin; C-C motif chemokine 11; Eosinophil chemotactic protein; Small-inducible cytokine A11

Species

Rattus norvegicus (Rat)

Source

E.coli

Expression Region

24-97aa

Complete Sequence

HPGSIPTSCC FTMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTKLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP

Mol. Weight

8.4 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CCL11 (Eotaxin) is a potent eosinophil-selective chemokine central to allergic inflammation and tissue eosinophilia, making precise recombinant forms essential for dissecting CC chemokine receptor 3 (CCR3) signaling. This tag-free rat CCL11 encompasses the full-length mature sequence (residues 24–97) and demonstrates confirmed biological activity in eosinophil chemotaxis bioassays at 0.1–1.0 μg/ml, supporting use in migration and transwell assays, CCR3-dependent MAPK and calcium flux signaling studies, and in vivo rat models of allergic airway inflammation. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound eosinophil activation readouts and mast cell degranulation controls, which aligns with standards expected in sensitive immune cell functional assays. Purity exceeding 96% by SDS-PAGE, combined with the low endotoxin profile, provides a suitable basis for use as a reference standard in cytokine detection assays and as a coating antigen for anti-rat Eotaxin antibody validation by ELISA.

Target Background

Function

In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils. Binds to CCR3.

Gene References into Functions

our data suggest that the CCL11-CCR3 interaction mainly activates PI3K/Akt signal transduction pathway in HUVECs. PMID: 28279120
Iddm30 controls pancreatic expression of Ccl11 (Eotaxin) and the Th1/Th2 balance within the insulitic lesions. PMID: 24646746
Tripterine can inhibit expression of Eotaxin by restraining the activation of NF-kappaB in the nasal musoca of rats with allergic rhinitis. PMID: 23272499
expression of Ccl11 associates with immune response modulation in experimental autoimmune encephalomyelitis PMID: 22815714
Intervention of PRMT1 activity can abrogate IL-4-dependent eotaxin-1 production to influence the pulmonary inflammation with eosinophil infiltration. PMID: 22387551
The expression of Eotaxin in nasal lavage fluid and nasal mucosa increased after treatment of allergic rhinitis with histamine. PMID: 20359111
The expression of Eotaxin in nasal mucosa increased, and the expression of CCR3 in bone marrow increased in the model of allergic rhinitis. PMID: 16722399
Suppression of eotaxin by CpG DNA reduced the symptoms of experimental allergic rhinitis. PMID: 16838745
Targeting CCL11 with monoclonal antibodies possibly results in decreased myocardial fibrosis and improves contractile function after heart transplantation ub rats. PMID: 20832583
Galectin-3 inhibited expression of eotaxin and IL5, potentially suppressing eosinophilic inflammation in asthma. PMID: 17074272
Eotaxin is upregulated in the pancreatic lymph nodes and may play a role in early disease pathogenesis of type 1 diabetes mellitus in the BioBreeding rat model. PMID: 15557196
In a model of ongoing acute heart transplant rejection in the rat, we found eotaxin mRNA expression within infiltrating macrophages, but not in mast cells PMID: 19631640

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Subcellular Location

Secreted.

Protein Families

Intercrine beta (chemokine CC) family

Database Links

KEGG: rno:29397

STRING: 10116.ENSRNOP00000009756

UniGene: Rn.10632

Code	CSB-AP001561RA
Abbreviation	Recombinant Rat Scya11 protein (Active)
MSDS	MSDS Datasheet
Size	$142
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Recombinant Rat Eotaxin protein (Ccl11) (Active)

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Target Background

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