Recombinant Rat Eotaxin protein(Ccl11) (Active)

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Code CSB-AP001561RA
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Product Details

Purity >96% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 0.1-1.0 μg/ml.
Target Names Ccl11
Uniprot No. P97545
Research Area Immunology
Alternative Names Ccl11; Scya11Eotaxin; C-C motif chemokine 11; Eosinophil chemotactic protein; Small-inducible cytokine A11
Species Rattus norvegicus (Rat)
Source E.coli
Expression Region 24-97aa
Complete Sequence HPGSIPTSCC FTMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTKLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP
Mol. Weight 8.4 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 5-10 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils. Binds to CCR3.
Gene References into Functions
  1. our data suggest that the CCL11-CCR3 interaction mainly activates PI3K/Akt signal transduction pathway in HUVECs. PMID: 28279120
  2. Iddm30 controls pancreatic expression of Ccl11 (Eotaxin) and the Th1/Th2 balance within the insulitic lesions. PMID: 24646746
  3. Tripterine can inhibit expression of Eotaxin by restraining the activation of NF-kappaB in the nasal musoca of rats with allergic rhinitis. PMID: 23272499
  4. expression of Ccl11 associates with immune response modulation in experimental autoimmune encephalomyelitis PMID: 22815714
  5. Intervention of PRMT1 activity can abrogate IL-4-dependent eotaxin-1 production to influence the pulmonary inflammation with eosinophil infiltration. PMID: 22387551
  6. The expression of Eotaxin in nasal lavage fluid and nasal mucosa increased after treatment of allergic rhinitis with histamine. PMID: 20359111
  7. The expression of Eotaxin in nasal mucosa increased, and the expression of CCR3 in bone marrow increased in the model of allergic rhinitis. PMID: 16722399
  8. Suppression of eotaxin by CpG DNA reduced the symptoms of experimental allergic rhinitis. PMID: 16838745
  9. Targeting CCL11 with monoclonal antibodies possibly results in decreased myocardial fibrosis and improves contractile function after heart transplantation ub rats. PMID: 20832583
  10. Galectin-3 inhibited expression of eotaxin and IL5, potentially suppressing eosinophilic inflammation in asthma. PMID: 17074272
  11. Eotaxin is upregulated in the pancreatic lymph nodes and may play a role in early disease pathogenesis of type 1 diabetes mellitus in the BioBreeding rat model. PMID: 15557196
  12. In a model of ongoing acute heart transplant rejection in the rat, we found eotaxin mRNA expression within infiltrating macrophages, but not in mast cells PMID: 19631640

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Subcellular Location Secreted.
Protein Families Intercrine beta (chemokine CC) family
Database Links

KEGG: rno:29397

STRING: 10116.ENSRNOP00000009756

UniGene: Rn.10632

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