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Rat Eotaxin 1 (Eotaxin 1/CCL11/ECF)ELISA Kit

Citations (1) Protocol
Code CSB-E07319r
Size 96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name chemokine (C-C motif) ligand 11
Alternative Names Ccl11 ELISA Kit; Scya11Eotaxin ELISA Kit; C-C motif chemokine 11 ELISA Kit; Eosinophil chemotactic protein ELISA Kit; Small-inducible cytokine A11 ELISA Kit
Abbreviation CCL11
Uniprot No. P97545
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/ml -1000 pg/ml
Sensitivity 3.9 pg/ml
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Eotaxin 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 99  
Range % 92-103  
1:2 Average % 88  
Range % 82-92  
1:4 Average % 91  
Range % 82-95  
1:8 Average % 93  
Range % 87-98  
Recovery
The recovery of rat Eotaxin 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 84-93  
EDTA plasma (n=4) 103 93-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.106 2.178 2.142 1.972  
5 1.479 1.445 1.462 1.292  
2.5 1.015 1.001 1.008 0.838  
1.25 0.644 0.620 0.632 0.462  
0.625 0.442 0.434 0.438 0.268  
0.312 0.332 0.327 0.330 0.160  
0.156 0.256 0.247 0.252 0.082  
0 0.171 0.169 0.170    
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils. Binds to CCR3.
Gene References into Functions
  1. our data suggest that the CCL11-CCR3 interaction mainly activates PI3K/Akt signal transduction pathway in HUVECs. PMID: 28279120
  2. Iddm30 controls pancreatic expression of Ccl11 (Eotaxin) and the Th1/Th2 balance within the insulitic lesions. PMID: 24646746
  3. Tripterine can inhibit expression of Eotaxin by restraining the activation of NF-kappaB in the nasal musoca of rats with allergic rhinitis. PMID: 23272499
  4. expression of Ccl11 associates with immune response modulation in experimental autoimmune encephalomyelitis PMID: 22815714
  5. Intervention of PRMT1 activity can abrogate IL-4-dependent eotaxin-1 production to influence the pulmonary inflammation with eosinophil infiltration. PMID: 22387551
  6. The expression of Eotaxin in nasal lavage fluid and nasal mucosa increased after treatment of allergic rhinitis with histamine. PMID: 20359111
  7. The expression of Eotaxin in nasal mucosa increased, and the expression of CCR3 in bone marrow increased in the model of allergic rhinitis. PMID: 16722399
  8. Suppression of eotaxin by CpG DNA reduced the symptoms of experimental allergic rhinitis. PMID: 16838745
  9. Targeting CCL11 with monoclonal antibodies possibly results in decreased myocardial fibrosis and improves contractile function after heart transplantation ub rats. PMID: 20832583
  10. Galectin-3 inhibited expression of eotaxin and IL5, potentially suppressing eosinophilic inflammation in asthma. PMID: 17074272
  11. Eotaxin is upregulated in the pancreatic lymph nodes and may play a role in early disease pathogenesis of type 1 diabetes mellitus in the BioBreeding rat model. PMID: 15557196
  12. In a model of ongoing acute heart transplant rejection in the rat, we found eotaxin mRNA expression within infiltrating macrophages, but not in mast cells PMID: 19631640

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Subcellular Location Secreted.
Protein Families Intercrine beta (chemokine CC) family
Database Links

KEGG: rno:29397

STRING: 10116.ENSRNOP00000009756

UniGene: Rn.10632
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