Rat Eotaxin 1 (Eotaxin 1/CCL11/ECF)ELISA Kit

Code CSB-E07319r
Size 96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
chemokine (C-C motif) ligand 11
Alternative Names
Ccl11 ELISA Kit; Scya11Eotaxin ELISA Kit; C-C motif chemokine 11 ELISA Kit; Eosinophil chemotactic protein ELISA Kit; Small-inducible cytokine A11 ELISA Kit
Abbreviation
CCL11
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 pg/ml -1000 pg/ml
Sensitivity
3.9 pg/ml
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Eotaxin 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 99  
Range % 92-103  
1:2 Average % 88  
Range % 82-92  
1:4 Average % 91  
Range % 82-95  
1:8 Average % 93  
Range % 87-98  
Recovery
The recovery of rat Eotaxin 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 84-93  
EDTA plasma (n=4) 103 93-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.106 2.178 2.142 1.972  
5 1.479 1.445 1.462 1.292  
2.5 1.015 1.001 1.008 0.838  
1.25 0.644 0.620 0.632 0.462  
0.625 0.442 0.434 0.438 0.268  
0.312 0.332 0.327 0.330 0.160  
0.156 0.256 0.247 0.252 0.082  
0 0.171 0.169 0.170    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils. Binds to CCR3.
Gene References into Functions
  1. our data suggest that the CCL11-CCR3 interaction mainly activates PI3K/Akt signal transduction pathway in HUVECs. PMID: 28279120
  2. Iddm30 controls pancreatic expression of Ccl11 (Eotaxin) and the Th1/Th2 balance within the insulitic lesions. PMID: 24646746
  3. Tripterine can inhibit expression of Eotaxin by restraining the activation of NF-kappaB in the nasal musoca of rats with allergic rhinitis. PMID: 23272499
  4. expression of Ccl11 associates with immune response modulation in experimental autoimmune encephalomyelitis PMID: 22815714
  5. Intervention of PRMT1 activity can abrogate IL-4-dependent eotaxin-1 production to influence the pulmonary inflammation with eosinophil infiltration. PMID: 22387551
  6. The expression of Eotaxin in nasal lavage fluid and nasal mucosa increased after treatment of allergic rhinitis with histamine. PMID: 20359111
  7. The expression of Eotaxin in nasal mucosa increased, and the expression of CCR3 in bone marrow increased in the model of allergic rhinitis. PMID: 16722399
  8. Suppression of eotaxin by CpG DNA reduced the symptoms of experimental allergic rhinitis. PMID: 16838745
  9. Targeting CCL11 with monoclonal antibodies possibly results in decreased myocardial fibrosis and improves contractile function after heart transplantation ub rats. PMID: 20832583
  10. Galectin-3 inhibited expression of eotaxin and IL5, potentially suppressing eosinophilic inflammation in asthma. PMID: 17074272
  11. Eotaxin is upregulated in the pancreatic lymph nodes and may play a role in early disease pathogenesis of type 1 diabetes mellitus in the BioBreeding rat model. PMID: 15557196
  12. In a model of ongoing acute heart transplant rejection in the rat, we found eotaxin mRNA expression within infiltrating macrophages, but not in mast cells PMID: 19631640

Show More

Hide All

Subcellular Location
Secreted.
Protein Families
Intercrine beta (chemokine CC) family
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 322 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1