AADB Antibody

Code CSB-PA360035XA01ENL
Size US$299
Image
  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: AADB antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 24.9 kDa
    Observed band size: 28 kDa
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Product Details

Full Product Name
Rabbit anti-Escherichia coli aadB Polyclonal antibody
Uniprot No.
Target Names
aadB
Alternative Names
aadB2''-aminoglycoside nucleotidyltransferase antibody; EC 2.7.7.46 antibody; AAD(2'') antibody; Gentamicin 2''-nucleotidyltransferase antibody; Gentamicin resistance protein antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli
Immunogen
Recombinant Escherichia coli AADB protein
Immunogen Species
Escherichia coli
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The recombinant Escherichia coli AADB protein is introduced into a rabbit, which triggers the production of antibodies that specifically target this antigen. The antibody production is further enhanced through a booster injection. The resulting AADB polyclonal antibodies are then collected from the rabbit's serum and purified using protein A/G. Rigorous testing through ELISA and WB assays has confirmed that the AADB antibody specifically recognizes the Escherichia coli AADB protein.

The Escherichia coli AADB protein, also known as the Aminoglycoside 2'-N-acetyltransferase, is to confer resistance to aminoglycoside antibiotics. It accomplishes this by modifying the antibiotics, specifically by acetylating the 2'-hydroxyl group of aminoglycosides, which reduces their binding affinity to the bacterial ribosome and prevents their antibacterial action.

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Target Background

Function
Mediates bacterial resistance to kanamycin, gentamicin and tobramycin by adenylating the 2''-hydroxyl group of these antibiotics.
Database Links

KEGG: ag:CAA28209

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