AADB Antibody

^{Rare Species}

Datasheet

Code	CSB-PA360035XA01ENL
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: AADB antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 24.9 kDa Observed band size: 28 kDa
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Product Details

Full Product Name

Rabbit anti-Escherichia coli aadB Polyclonal antibody

Uniprot No.

P0AE04

Target Names

aadB

Alternative Names

aadB2''-aminoglycoside nucleotidyltransferase antibody; EC 2.7.7.46 antibody; AAD(2'') antibody; Gentamicin 2''-nucleotidyltransferase antibody; Gentamicin resistance protein antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli

Immunogen

Recombinant Escherichia coli AADB protein

Immunogen Species

Escherichia coli

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein A/G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The recombinant Escherichia coli AADB protein is introduced into a rabbit, which triggers the production of antibodies that specifically target this antigen. The antibody production is further enhanced through a booster injection. The resulting AADB polyclonal antibodies are then collected from the rabbit's serum and purified using protein A/G. Rigorous testing through ELISA and WB assays has confirmed that the AADB antibody specifically recognizes the Escherichia coli AADB protein.

The Escherichia coli AADB protein, also known as the Aminoglycoside 2'-N-acetyltransferase, is to confer resistance to aminoglycoside antibiotics. It accomplishes this by modifying the antibiotics, specifically by acetylating the 2'-hydroxyl group of aminoglycosides, which reduces their binding affinity to the bacterial ribosome and prevents their antibacterial action.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Mediates bacterial resistance to kanamycin, gentamicin and tobramycin by adenylating the 2''-hydroxyl group of these antibiotics.

Database Links

KEGG: ag:CAA28209

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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AADB Antibody

Product Details

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Customer Reviews and Q&A

Target Background