Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AGO2 Polyclonal antibody

Uniprot No.

Q9SHF3

Target Names

AGO2

Alternative Names

AGO2 antibody; At1g31280 antibody; T19E23.7Protein argonaute 2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana AGO2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Involved in RNA-mediated post-transcriptional gene silencing (PTGS). Main component of the RNA-induced silencing complex (RISC) that binds to a short guide RNA such as microRNA (miRNA) or small interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide for slicer-directed cleavage of homologous mRNAs to repress gene expression. Associates mainly with siRNAs of 21 nucleotide in length and preferentially recruits small RNAs with a 5' terminal adenosine. Probably involved in antiviral RNA silencing. Associates with siRNA derived from cucumber mosaic virus (CMV). Targeted by turnip yellows virus (TuYV) protein P0 (via F-box-like domain) for probable proteasome degradation and thereby inactivating AGO2 function in RNA silencing. Required to direct NERD-dependent DNA methylation and silencing.

Gene References into Functions

Data show that Argonaute family protein AGO2 and AGO3 were important for abscisic acid (ABA)-mediated resistance. PMID: 28270624
Base pairing at the 15th nucleotide of a miRNA duplex is important for miRNA sorting in both Arabidopsis AGO1 and AGO2. AGO2 favours miRNA duplexes with no middle mismatches. PMID: 25406978
Complementation analyses in ago mutant plants revealed that the catalytic residues of AGO1, AGO2, and AGO7 are required to restore the defects of Arabidopsis ago1-25, ago2-1, and zip-1 (AGO7-defective) mutants, respectively. PMID: 23023169
AGO protein has evolved to specialize in antiviral defenses. PMID: 21576511
AGO1 and AGO2 are involved in defense against mutant of Cucumber mosaic virus, which act downstream the biogenesis of viral secondary siRNAs in a nonredundant and cooperative manner. PMID: 21467580
AGO2 may regulate and coordinate the expression of a group of genes involved in various pathways of plant immunity by binding to a group of functional sRNAs. [AGO2] [Argonaute 2] PMID: 21549312
second layer is activated when the first layer is suppressed because AGO2 is repressed by AGO1 via miR403 PMID: 21305057

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Protein Families

Argonaute family, Ago subfamily

Database Links

KEGG: ath:AT1G31280

STRING: 3702.AT1G31280.1

UniGene: At.40380

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AGO2 Antibody

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