AGO2 Antibody

1. Results provide evidence for an Ago2-interaction network participating in dosage compensation in drosophila. PMID: 29921620
2. Molecular evolution of AGO2 glutamine-rich repeat region PMID: 27317784
3. Mutation of T1149 or R1158 in the conserved PIWI domain causes AGO2 protein instability, but only T1149 affects RNAi activity. Mass spec analysis shows that several proteasome components co-purify with both wildtype and mutant AGO2, and knockdown of two proteasome pathway components results in AGO2 protein accumulation. PMID: 28087276
4. We speculate that the repeated evolution of testis specificity in obscura group Ago2 genes, combined with their dynamic turnover and strong signatures of adaptive evolution, may be associated with highly derived roles in the suppression of transposable elements or meiotic drive PMID: 27535930
5. Study shows that the Cricket Paralysis virus suppressor of RNA silencing, CrPV-1A but not B2 strongly interfere with the Ago-2-dependent miRNA silencing in Drosophila. PMID: 25793377
6. siRNA biogenesis does not stabilize AGO2 in Drosophila. PMID: 23708604
7. The results of this study found that mutations in Drosophila Argonaute 2 (Ago2) resulted in exacerbated transposon expression in the brain, progressive and age-dependent memory impairment, and shortened lifespan PMID: 23563579
8. two new nuclear roles for Ago-2: one in pre-mRNA splicing and one in transcriptional repression. PMID: 23392611
9. Highlighting its role in antiviral defense, fly Ago2 dissociates so slowly from extensively complementary target RNAs that essentially every fully paired target is cleaved. Conversely, mouse AGO2, which mainly mediates miRNA-directed repression, dissociates rapidly and with similar rates for fully paired and seed-matched targets. PMID: 23178124
10. Nora virus VP1 and the viral suppressor of RNAi of Cricket paralysis virus (1A) antagonized Argonaute-2 (AGO2) Slicer activity of RNA induced silencing complexes pre-loaded with a methylated single-stranded guide strand. PMID: 22916019
11. GRR variation does not modulate an essential function of Ago2 and the amino-terminal domain of Ago2 is subject to rapid evolution PMID: 21253006
12. Data suggest that recent independent selective sweeps in AGO2 have reduced genetic variation across a region of more than 50 kbp in Drosophila melanogaster, D. simulans, and D. yakuba. PMID: 20978039
13. three-dimensional nuclear magnetic resonance structure of the Drosophila melanogaster Ago2 PAZ domain PMID: 14615801
14. Crystal structure of the PAZ domain PMID: 14625589
15. describe the solution structures of the Argonaute2 PAZ domain bound to RNA and DNA oligonucleotides PMID: 15156196
16. NMR assignment of the Drosophila Argonaute2 PAZ domain PMID: 15213449
17. AGO2 is an essential component for siRNA-directed RNA interference (RNAi) response and is required for the unwinding of siRNA duplex and in consequence assembly of siRNA into RISC in Drosophila embryos. PMID: 15231716
18. found homology between the PIWI domain of Ago-2 and endonuclease V and identified potential active-site amino acid residues within the PIWI domain of Ago-2 PMID: 15452342
19. Depletion of AGO2, the essentiual factor for siRNA-directed RNAi, resulted in EFGP mRNA stabilization with concomitant shortening of the EFGP mRNA poly(A)tail. PMID: 15987811
20. nuclear division is asynchronous in mutant ago-2 embryos and there are defects in chromosome condensation, nuclear kinesis, and assembly of spindle apparatus PMID: 16024657
21. Results show both strands of siRNA get loaded onto Ago2 protein in RNA interference. PMID: 16271385
22. Ago2 is the catalytic component of the RNAi enzyme complex (RISC) and directly receives the double-stranded siRNA from the RISC assembly machinery. PMID: 16271386
23. A genome-wide analysis of mRNAs regulated by AGO2 was performed. PMID: 16581772
24. Argonaute 2 is essential for antiviral defense in adult Drosophila melanogaster. PMID: 17079687
25. all Argonautes elevate mature miRNA expression posttranscriptionally, independent of RNase activity. we identify a role for (Ago2) in cleaving the pre-miRNA to an additional processing intermediate, termed Ago2-cleaved precursor miRNA PMID: 18083100
26. Sequence requirements for loading small RNAs into functional Argonaute complexes narrow the range of miRNA and miRNA 5' ends that accumulate in flies. PMID: 18207740
27. findings indicate that different types of small RNAs and Argonautes are used to repress retrotransposons in germline and somatic cells in Drosophila PMID: 18463636
28. processing of cis-NATs by RNA interference (RNAi) seems to be actively restricted, and the selected loci are enriched for nucleic acid-based functions and include Argonaute-2 (AGO2) itself PMID: 18500351
29. Ago2-RISC represses translation by competitively blocking the interaction of eIF4E with eIF4G and inhibiting the cap function. PMID: 19268617

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KEGG: dme:Dmel_CG7439

STRING: 7227.FBpp0075313

UniGene: Dm.9677