ALG11 Antibody

Code CSB-PA894498XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ALG11 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
ALG11; LEW3; At2g40190; T07M07.7; GDP-Man:Man(3GlcNAc(2-PP-Dol alpha-1,2-mannosyltransferase; Alpha-1,2-mannosyltransferase ALG11; Asparagine-linked glycosylation protein 11; Protein LEAF WILTING 3
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ALG11 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for N-linked oligosaccharide assembly. Has a role in the last step of the synthesis of the Man(5)GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum.
Gene References into Functions
  1. Positional cloning determined that lrg1 affects an alpha-1,2-mannosyltransferase gene, LEW3, involved in protein N-glycosylation. The lrg1 mutation causes a synonymous substitution that alters the correct splicing of the fourth intron in LEW3, causing a mix of wild-type and truncated protein. LRG1 RNA missplicing in roots and short root phenotypes in lrg1 are light-intensity dependent. PMID: 29106622
  2. The lew3 mutation reduces protein glycosylation, impairs cellulose synthesis, and results in xylem collapse. lew3 mutants are more sensitive to salt stress and transpire less water than the wild-type. PMID: 19732381
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
Glycosyltransferase 4 family
Tissue Specificity
Expressed in shoots, leaves, sepals, filaments, siliques, stems, roots and guard cells.
Database Links

KEGG: ath:AT2G40190

STRING: 3702.AT2G40190.1

UniGene: At.21597

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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