Atg5 Antibody

Code CSB-PA895235XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Atg5 Polyclonal antibody
Uniprot No.
Target Names
Atg5
Alternative Names
Atg5 antibody; CG1643Autophagy protein 5 antibody; APG5-like antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Atg5 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in autophagic vesicle formation. Conjugation with Atg12, through a ubiquitin-like conjugating system involving Atg7 as an E1-like activating enzyme and Atg10 as an E2-like conjugating enzyme, is essential for its function. The Atg12-Atg5 conjugate acts as an E3-like enzyme which is required for lipidation of Atg8 and its association to the vesicle membranes.
Gene References into Functions
  1. Studies show that the TOR signaling pathway and ATG5 and ATG7 are crucial for the regulation of cellular processes and can play various functions in different cell types in the brain. PMID: 28196106
Subcellular Location
Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein.
Protein Families
ATG5 family
Database Links

KEGG: dme:Dmel_CG1643

STRING: 7227.FBpp0071023

UniGene: Dm.33486

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