Atp5f1b Antibody

Code CSB-PA002350ZA01RA
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Product Details

Full Product Name
Rabbit anti-Rattus norvegicus (Rat) Atp5f1b Polyclonal antibody
Uniprot No.
Target Names
Atp5f1b
Alternative Names
ATP synthase subunit beta, mitochondrial (EC 3.6.3.14), Atp5b
Raised in
Rabbit
Species Reactivity
Rattus norvegicus
Immunogen
Recombinant Rattus norvegicus Atp5f1b protein
Immunogen Species
Rattus norvegicus (Rat)
Conjugate
Non-conjugate
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)

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Target Background

Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Gene References into Functions
  1. Hyperglycemia may impair mitochondrial function and alter glycolysis in glomerular epithelial cells by down-regulating the expression of ATP synthase beta subunit and enolase 2. PMID: 24042457
  2. Increased mitochondrial energy metabolism efficiency via F1F0 ATPase synthase beta subunit contributes to the enhanced synaptic efficacy found in Bcl-x(L)-expressing neurons. PMID: 21926988
  3. data provides evidence that the F1-ATPase beta-subunit is the enterostatin receptor and suggests that enterostatin and beta-casomorphin1-7 bind to distinct sites on the protein PMID: 15572201
  4. In the absence of PKG activity, we propose that some ATP synthasebeta precursor dissociates from 14-3-3sigma, resulting in its activation and incorporation into the ATP synthase complex, which results in an increase in ATP and apoptosis PMID: 17303654
  5. consumption of soy proteins increases hepatic ATPase activity, which might be a consequence of increased dephosphorylation or decreased phosphorylation of the mitochondrial ATPase/ATP synthase beta protein PMID: 17709438
  6. study shows that the beta-subunit of F1-ATPase is ectopically expressed in the plasma membrane of INS-1 cells and that the appetite regulating peptide enterostatin and fatty acids cause an up-regulation of this protein PMID: 18510804

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Subcellular Location
Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Protein Families
ATPase alpha/beta chains family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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