ATP5F1B

ATP5F1B encodes the ATP synthase F1 subunit beta, mitochondrial (ATP5B), also known as the F1-ATPase beta subunit. This protein is a core component of the F1 catalytic domain of mitochondrial ATP synthase, which generates ATP from ADP and inorganic phosphate via oxidative phosphorylation. It undergoes conformational changes driven by proton flow across the inner mitochondrial membrane, enabling ATP synthesis—the primary energy source for cellular processes.
ATP5F1B is central to the oxidative phosphorylation pathway. Mutations in this gene cause mitochondrial dysfunction, linked to disorders like Leigh syndrome and mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS). While direct targeted drugs are still in preclinical stages, research focuses on metabolic modulators and gene therapies to correct ATP synthase defects, aiming to alleviate symptoms of associated mitochondrial diseases.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

ATP5B Recombinant Monoclonal Antibody (CSB-RA216446A0HU)

Validated Data

Western Blot
Positive WB detected in: 293T whole cell lysate, HT29 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, Rat Heart tissue, Mouse Heart tissue
All lanes: ATP5F1B antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa

IHC image of CSB-RA216446A0HU diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA216446A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

ATP5B Antibody (CSB-PA002350EA01HU)

Validated Data

Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Mouse heart tissue, Mouse liver tissue, Mouse brain tissue, Rat heart tissue, Rat liver tissue
All lanes: ATP5B antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA002350EA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA002350EA01HU at dilution of 1:100

Human ATP synthase subunit beta, mitochondrial(ATP5B) ELISA kit (CSB-EL002350HU)

Validated Data

Code: CSB-EL002350HU

Size: 96T,5×96T,10×96T

Sensitivity: 6.25 pg/mL

Detection Range: 25 pg/mL-1600 pg/mL

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

The following ATP5F1B reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

ATP5F1B Antibodies

ATP5F1B Proteins

ATP5F1B ELISA Kits

ATP5F1B Antibodies

ATP5F1B Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA002350GA01HU	ATP5B Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA002350ESR2HU	ATP5B Antibody	Human	ELISA, WB, IHC
CSB-PA002350EA01HU	ATP5B Antibody	Human, Mouse, Rat	ELISA, WB, IHC
CSB-RA216446A0HU	ATP5B Recombinant Monoclonal Antibody	Human, Mouse, Rat	ELISA, WB, IHC

ATP5F1B Proteins

ATP5F1B Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP002350HU	Recombinant Human ATP synthase F(1) complex subunit beta, mitochondrial (ATP5F1B)	Yeast
CSB-BP002350HU CSB-MP002350HU CSB-EP002350HU-B	Recombinant Human ATP synthase F(1) complex subunit beta, mitochondrial (ATP5F1B)	Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP002350HU1	Recombinant Human ATP synthase F(1) complex subunit beta, mitochondrial (ATP5F1B), partial	E.coli
CSB-EP002350HU	Recombinant Human ATP synthase F(1) complex subunit beta, mitochondrial (ATP5F1B)	E.coli

ATP5F1B ELISA Kit

ATP5F1B ELISA Kit for Homo sapiens (Human)

Code	Product Name	Sample Types	Sensitivity
CSB-EL002350HU	Human ATP synthase subunit beta, mitochondrial(ATP5B) ELISA kit	serum, plasma, tissue homogenates, cell lysates	6.25 pg/mL

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ATP5F1B

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