bub1 Antibody

Code CSB-PA528509XA01SXV
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Product Details

Full Product Name
Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) bub1 Polyclonal antibody
Uniprot No.
Target Names
bub1
Alternative Names
bub1 antibody; SPCC1322.12cCheckpoint serine/threonine-protein kinase bub1 antibody; EC 2.7.11.1 antibody
Raised in
Rabbit
Species Reactivity
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Immunogen
Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) bub1 protein
Immunogen Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in cell cycle checkpoint enforcement. Acts to stabilize the spindle during mitosis. Required for the correct localization of bub3 and mad3 to the kinetochore. Appears to have a role in chromosome segregation. Catalyzes the phosphorylation of bub3.
Gene References into Functions
  1. Study shows that fission yeast Moa1 (meikin), which associates stably with CENP-C during meiosis I, recruits Plo1 (polo-like kinase) to the kinetochores and phosphorylates Spc7 (KNL1) to accumulate Bub1. Consequently, in contrast to the transient kinetochore localization of mitotic Bub1, meiotic Bub1 persists at kinetochores until anaphase I. PMID: 28497540
  2. findings show phosphorylation of histone H3-threonine 3 mediated by Haspin cooperates with Bub1-mediated histone 2A-serine 121 phosphorylation in targeting the chromosomal passenger complex to the inner centromere in fission yeast and human cells PMID: 20929775
  3. findings show that Bub1 phosphorylates the conserved serine 121 of histone H2A; Bub1 kinase creates a mark for shugoshin localization and the correct partitioning of chromosomes PMID: 19965387
  4. role for the highly conserved N-terminal domain of Bub1p in the robust targeting of Bub1p, Bub3p, and Mad3p to kinetochores PMID: 15509783
  5. Bub1p-A78V is defective in both nuclear accumulation and kinetochore targeting; a threshold level of nuclear Bub1p is necessary for the nuclear accumulation of Bub3p and Mad3p PMID: 15525673
  6. The N terminus of Bub1 is necessary and sufficient for Sgo1 targeting to centromeres and the protection of cohesion during meiosis. PMID: 16360688
  7. A novel temperature-sensitive mutant of fission yeast alpha-tubulin Atb2 (atb2-983) displays a high rate of chromosome missegregation and is synthetically lethal with deletions in a subset of spindle checkpoint genes including bub1. PMID: 16394105
  8. Sgo2, which assists in loading Aurora to centromeres, and its regulator Bub1 are required for the mono-orientation of sister chromatids in meiosis-I, while Ark1 is required for the faithful bi-orientation PMID: 17932486
  9. Bub1 stably binds kinetochores, and by tethering Bub1 to telomeres we demonstrate that it is sufficient to recruit anaphase inhibitors in a kinase-independent manner. We propose that the major checkpoint role for Bub1 is as a signalling scaffold PMID: 18094750
  10. analysis of dual regulation of Mad2 localization on kinetochores by Bub1 and Dam1/DASH that ensure proper spindle interaction PMID: 18632983
  11. find that Bub3 is also dispensable for shugoshin localization to the centromeres, which is the second known function of Bub1. PMID: 19680287

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Subcellular Location
Nucleus. Chromosome, centromere, kinetochore. Chromosome, centromere. Note=Associates with kinetochores and centromeres during the early stages of mitosis.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, BUB1 subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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