Recombinant Schizosaccharomyces pombe Checkpoint serine/threonine-protein kinase bub1 (bub1), partial

Code CSB-YP528509SXV
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Source Yeast
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Code CSB-EP528509SXV
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Source E.coli
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Code CSB-EP528509SXV-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP528509SXV
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Source Baculovirus
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Code CSB-MP528509SXV
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
bub1
Uniprot No.
Alternative Names
bub1; SPCC1322.12cCheckpoint serine/threonine-protein kinase bub1; EC 2.7.11.1
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in cell cycle checkpoint enforcement. Acts to stabilize the spindle during mitosis. Required for the correct localization of bub3 and mad3 to the kinetochore. Appears to have a role in chromosome segregation. Catalyzes the phosphorylation of bub3.
Gene References into Functions
  1. Study shows that fission yeast Moa1 (meikin), which associates stably with CENP-C during meiosis I, recruits Plo1 (polo-like kinase) to the kinetochores and phosphorylates Spc7 (KNL1) to accumulate Bub1. Consequently, in contrast to the transient kinetochore localization of mitotic Bub1, meiotic Bub1 persists at kinetochores until anaphase I. PMID: 28497540
  2. findings show phosphorylation of histone H3-threonine 3 mediated by Haspin cooperates with Bub1-mediated histone 2A-serine 121 phosphorylation in targeting the chromosomal passenger complex to the inner centromere in fission yeast and human cells PMID: 20929775
  3. findings show that Bub1 phosphorylates the conserved serine 121 of histone H2A; Bub1 kinase creates a mark for shugoshin localization and the correct partitioning of chromosomes PMID: 19965387
  4. role for the highly conserved N-terminal domain of Bub1p in the robust targeting of Bub1p, Bub3p, and Mad3p to kinetochores PMID: 15509783
  5. Bub1p-A78V is defective in both nuclear accumulation and kinetochore targeting; a threshold level of nuclear Bub1p is necessary for the nuclear accumulation of Bub3p and Mad3p PMID: 15525673
  6. The N terminus of Bub1 is necessary and sufficient for Sgo1 targeting to centromeres and the protection of cohesion during meiosis. PMID: 16360688
  7. A novel temperature-sensitive mutant of fission yeast alpha-tubulin Atb2 (atb2-983) displays a high rate of chromosome missegregation and is synthetically lethal with deletions in a subset of spindle checkpoint genes including bub1. PMID: 16394105
  8. Sgo2, which assists in loading Aurora to centromeres, and its regulator Bub1 are required for the mono-orientation of sister chromatids in meiosis-I, while Ark1 is required for the faithful bi-orientation PMID: 17932486
  9. Bub1 stably binds kinetochores, and by tethering Bub1 to telomeres we demonstrate that it is sufficient to recruit anaphase inhibitors in a kinase-independent manner. We propose that the major checkpoint role for Bub1 is as a signalling scaffold PMID: 18094750
  10. analysis of dual regulation of Mad2 localization on kinetochores by Bub1 and Dam1/DASH that ensure proper spindle interaction PMID: 18632983
  11. find that Bub3 is also dispensable for shugoshin localization to the centromeres, which is the second known function of Bub1. PMID: 19680287

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Subcellular Location
Nucleus. Chromosome, centromere, kinetochore. Chromosome, centromere. Note=Associates with kinetochores and centromeres during the early stages of mitosis.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, BUB1 subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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