DERL2 Antibody

Code CSB-PA880924XA01HU
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) DERL2 Polyclonal antibody
Uniprot No.
Target Names
DERL2
Alternative Names
DERL2; DER2; FLANA; CGI-101; SBBI53; Derlin-2; Degradation in endoplasmic reticulum protein 2; DERtrin-2; Der1-like protein 2; F-LAN-1; F-LANa
Raised in
Rabbit
Species Reactivity
Homo sapiens (Human)
Immunogen
Recombinant Homo sapiens (Human) DERL2 protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Functional component of endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal glycoproteins, but not that of misfolded nonglycoproteins. May act by forming a channel that allows the retrotranslocation of misfolded glycoproteins into the cytosol where they are ubiquitinated and degraded by the proteasome. May mediate the interaction between VCP and misfolded glycoproteins. May also be involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation.; (Microbial infection) In contrast to DERL1, it is not involved in the degradation of MHC class I heavy chains following infection by cytomegaloviruses.
Gene References into Functions
  1. specific silencing of Derlin-2, p97 and HRD1 by shRNAs increases steady state levels of proinsulin. these ERAD constituents are critically involved in proinsulin degradation and may therefore also play a role in subsequent antigen generation. PMID: 26107514
  2. derlin2 functions with HRD1 in ERAD of certain substrates independent of their glycosylation status. PMID: 23867461
  3. Derlin-2 forms a robust multiprotein complex with the p97 AAA ATPase as well as the mammalian orthologs of the yeast Hrd1p/Hrd3p ubiquitin-ligase complex, and participates in the degradation of proteins from the ER. PMID: 16186509
  4. Findings indicate that Derlin-2 provides the missing link between EDEM and p97 in the process of degrading misfolded glycoproteins. PMID: 16449189
  5. Required for Polyoma virus infection. PMID: 16912321

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Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
Derlin family
Tissue Specificity
Ubiquitous. Overexpressed in various hepatocarcinomas.
Database Links

HGNC: 17943

OMIM: 610304

KEGG: hsa:51009

STRING: 9606.ENSP00000158771

UniGene: Hs.286131

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