Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DET1 Polyclonal antibody

Uniprot No.

P48732

Target Names

DET1

Alternative Names

DET1 antibody; At4g10180 antibody; F28M11.100 antibody; T9A4.13Light-mediated development protein DET1 antibody; Protein DEETIOLATED 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana DET1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Component of light signal transduction machinery. Involved in repression of photomorphogenesis in darkness by participating in the CDD complex, a complex probably required to regulate the activity of ubiquitin conjugating enzymes (E2s). Involved in repression of deetiolation in the developing seedling. Repression of photomorphogenesis is probably mediated by ubiquitination and subsequent degradation of photomorphogenesis-promoting factors such as HY5, HYH and LAF1. Involved in the repression of blue light responsive promoter in chloroplasts. May be required to stabilize the CDD complex. Its association with histone tail suggests a role in remodeling of chromatin. Required for the regulation of histone H2B monoubiquitination (H2Bub) over most genes by controlling the stability of the deubiquitination module (DUB module).

Gene References into Functions

Germination in det1 mutants is sensitive to ABA. Double mutant analysis indicates that det1 abscisic acid sensitive germination requires HY5 and ABI5. PMID: 26398801
DET1 is a strong repressor of flowering and has a pivotal role in maintaining photoperiod sensitivity in the regulation of flowering time. PMID: 25962685
PEX2 RING domain overexpression in det1 also partially suppresses the det1 phenotype. PMID: 25248106
Genomic analysis also revealed that DET1 may modulate the expression of light-regulated genes to mediate photomorphogenesis partially through phytochrome-interacting factors. PMID: 25248553
DET1 acts upstream of both HFR1 and PIF1 to predominantly repress seed germination. DET1 predominantly represses seed germination in the dark. PMID: 25775589
The det1 acts epistatically to uvh6 with respect to de-etiolation, chlorophyll level, UV tolerance, and heat-induced growth inhibition. (DET1 protein) PMID: 22908040
results reveal DET1 as a core transcriptional repression factor important for clock progression PMID: 21884973
Altogether, these data suggest that DET1, CUL4 and DDB2 cooperate during the excision repair process. PMID: 21304489
LHY ubiquitination by SINAT5 was inhibited by DET1. PMID: 20599732
Findings suggests a role for DET1 in a ubiquitination pathway and identify a substrate for DET1-regulated protein turn-over. PMID: 15988568
Arabidopsis cullin 4 is shown to assemble with DDB1, RBX1, DET1 and DDB2 in vitro and in planta. PMID: 16792691

Hide All

Subcellular Location

Nucleus, nucleoplasm.

Protein Families

DET1 family

Database Links

KEGG: ath:AT4G10180

STRING: 3702.AT4G10180.1

UniGene: At.33647

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DET1 Antibody

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