LIG1 Antibody

Code CSB-PA671465XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) LIG1 Polyclonal antibody
Uniprot No.
Target Names
LIG1
Alternative Names
LIG1 antibody; At1g08130 antibody; T23G18.1 antibody; T6D22.23DNA ligase 1 antibody; AtLIG1 antibody; EC 6.5.1.1 antibody; DNA ligase I antibody; Polydeoxyribonucleotide synthase [ATP] 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana LIG1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Essential protein. DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. Involved in repair of both single strand breaks (SSBs) and double strand breaks (DSBs). Required in the endosperm for embryogenesis, probably to repair DNA-breaks generated by DME.
Gene References into Functions
  1. The AtLIG1 is an important component of the active DNA demethylation machinery. PMID: 25906993
  2. LIG1 is required for both single-nucleotide or long-patch base excision repair. PMID: 21781197
  3. In the study, we show that loss-of-function of the major DNA LIGASE I (AtLIG1) in Arabidopsis thaliana causes maternal effects in the endosperm, which is the seed tissue that nurtures embryo development. PMID: 20023162
  4. Nuclear or mitochondrial targeting of LIG1 is accomplished through an evolutionarily conserved translation initiation mechanism. [AtLIG1] PMID: 16790030
  5. DNA ligase 1 functions in both DNA replication and in repair of both ss and dsDNA strand breaks. PMID: 19558640

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Subcellular Location
[Isoform 1]: Mitochondrion.; [Isoform 2]: Nucleus.; [Isoform 3]: Nucleus.
Protein Families
ATP-dependent DNA ligase family
Tissue Specificity
Expressed in all vegetative and reproductive tissues.
Database Links

KEGG: ath:AT1G08130

STRING: 3702.AT1G08130.1

UniGene: At.36

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