dlc-1 Antibody

Code CSB-PA637558XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans dlc-1 Polyclonal antibody
Uniprot No.
Target Names
dlc-1
Alternative Names
dlc-1 antibody; T26A5.9Dynein light chain 1 antibody; cytoplasmic antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans dlc-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts as a non-catalytic accessory component of a dynein complex. Part of a complex with bicd-1 and egal-1, which is recruited to the nuclear envelope by unc-83, where in turn, it recruits dynein to the nuclear surface and regulates nuclear migrations in hypodermal precursor cells. Probably within a dynein motor complex, plays a role in the cell fate specification of the germline and oogenesis. In particular, it inhibits germ cell proliferation. Regulates the function and localization of the RNA-binding protein fbf-2 in the germline. Plays a role in mitotic and meiotic processes. Involved in the pairing of homologous chromosomes. Independently of its dynein-mediated functions, plays a role in germ cell apoptosis.
Gene References into Functions
  1. DLC-1 directly binds to FBF-2 outside of the RNA-binding domain and promotes FBF-2 localization and function. This result identifies a new role for DLC-1 in post-transcriptional regulation of gene expression. PMID: 27864381
  2. DLC-1 is functioning cell nonautonomously through the same pathway as kri-1 in response to ionizing radiation-induced apoptosis. PMID: 24030151
  3. Dynein light chain 1 (DLC-1) and its partner, dynein heavy chain 1, inhibit the proliferative cell fate, in part through regulation of METT-10, a conserved putative methyltransferase. PMID: 19752194
Subcellular Location
Cytoplasm, cytoskeleton. Nucleus envelope. Cytoplasmic granule.
Protein Families
Dynein light chain family
Tissue Specificity
Broadly expressed in tissues including the intestine, body wall muscles, germs cells, oocytes, the rectal valve and cells in the head.
Database Links

KEGG: cel:CELE_T26A5.9

STRING: 6239.T26A5.9.2

UniGene: Cel.18125

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