Recombinant Dynein light chain 1, cytoplasmic (dlc-1)

Code CSB-YP637558CXY
MSDS
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Source Yeast
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Code CSB-EP637558CXY
MSDS
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Source E.coli
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Code CSB-EP637558CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP637558CXY
MSDS
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Source Baculovirus
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Code CSB-MP637558CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
dlc-1
Uniprot No.
Alternative Names
dlc-1; T26A5.9Dynein light chain 1; cytoplasmic
Species
Caenorhabditis elegans
Expression Region
1-89
Target Protein Sequence
MVDRKAVIKN ADMSDDMQQD AIDCATQALE KYNIEKDIAA YIKKEFDKKY NPTWHCIVGR NFGSYVTHET KHFIYFYLGQ VAILLFKSG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as a non-catalytic accessory component of a dynein complex. Part of a complex with bicd-1 and egal-1, which is recruited to the nuclear envelope by unc-83, where in turn, it recruits dynein to the nuclear surface and regulates nuclear migrations in hypodermal precursor cells. Probably within a dynein motor complex, plays a role in the cell fate specification of the germline and oogenesis. In particular, it inhibits germ cell proliferation. Regulates the function and localization of the RNA-binding protein fbf-2 in the germline. Plays a role in mitotic and meiotic processes. Involved in the pairing of homologous chromosomes. Independently of its dynein-mediated functions, plays a role in germ cell apoptosis.
Gene References into Functions
  1. DLC-1 directly binds to FBF-2 outside of the RNA-binding domain and promotes FBF-2 localization and function. This result identifies a new role for DLC-1 in post-transcriptional regulation of gene expression. PMID: 27864381
  2. DLC-1 is functioning cell nonautonomously through the same pathway as kri-1 in response to ionizing radiation-induced apoptosis. PMID: 24030151
  3. Dynein light chain 1 (DLC-1) and its partner, dynein heavy chain 1, inhibit the proliferative cell fate, in part through regulation of METT-10, a conserved putative methyltransferase. PMID: 19752194
Subcellular Location
Cytoplasm, cytoskeleton. Nucleus envelope. Cytoplasmic granule.
Protein Families
Dynein light chain family
Tissue Specificity
Broadly expressed in tissues including the intestine, body wall muscles, germs cells, oocytes, the rectal valve and cells in the head.
Database Links

KEGG: cel:CELE_T26A5.9

STRING: 6239.T26A5.9.2

UniGene: Cel.18125

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