Code | CSB-PA006847XA01ENV |
Size | US$299 |
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The folA polyclonal antibody production involves the repeated immunization of a rabbit with a recombinant Escherichia coli (strain K12) folA protein (1-159aa). When a sufficient titer (antibody concentration) is obtained, the rabbit is bled and the antibodies are purified from the serum through affinity chromatography. The functionality of the resulting folA antibody is tested in ELISA and WB applications. And it can react with Escherichia coli (strain K12) folA protein.
The Escherichia coli (strain K12) folA protein is a bifunctional enzyme that catalyzes two reactions: the reduction of dihydrofolate (DHF) to THF, and the conversion of 5,10-methylenetetrahydrofolate (5,10-methylene-THF) to 5-methyltetrahydrofolate (5-methyl-THF). This enzymatic reaction is a crucial step in the folate (vitamin B9) metabolic pathway. The activity of the folA protein is pivotal for providing the reduced form of folate needed for various biosynthetic processes within the cell.
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KEGG: ecj:JW0047
STRING: 316385.ECDH10B_0049