folA Antibody

^{Rare Species}

Datasheet

Code	CSB-PA006847XA01LMH
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes: folA Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa
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Target Background

Product Details

Uniprot No.

P00381

Target Names

folA

Alternative Names

Dihydrofolate reductase (EC 1.5.1.3) folA dhfR

Species Reactivity

Lactobacillus casei

Immunogen

Recombinant Lactobacillus casei folA protein (2-163aa)

Immunogen Species

Lactobacillus casei

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The procedure for generating polyclonal antibodies against folA involves repetitive immunizations of a rabbit with recombinant Lactobacillus casei folA protein (2-163aa). Upon reaching a sufficient antibody titer, the rabbit undergoes bleeding, and the serum-derived antibodies are purified through affinity chromatography. Subsequent testing of the folA antibody in ELISA and WB applications confirms its reactivity with Lactobacillus casei folA protein.

The main function of the Lactobacillus casei folA protein is to catalyze the conversion of dihydrofolate (DHF) into tetrahydrofolate (THF) through a process called dihydrofolate reductase activity. This enzyme is involved in folate metabolism, which is essential for the synthesis of nucleotides, amino acids, and other important cellular components. It plays a vital role in maintaining the balance of folate derivatives within the cell.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.

Protein Families

Dihydrofolate reductase family

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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folA Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background