Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) E2f2 Polyclonal antibody

Uniprot No.

O77051

Target Names

E2f2

Alternative Names

E2f2 antibody; CG1071Transcription factor E2F2 antibody; dE2F2 antibody; E2F transcription factor 2 antibody; E2F-like transcription factor E2F2 antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) E2f2 protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Transcriptional repressor that binds to E2f sites and represses E2f-regulated target genes. Binding to E2f sites requires transcription factor Dp. Acts synergistically with Rbf2 to antagonize E2f1-mediated transcriptional activation. Component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context. The DREAM complex is required for recruiting E2f2 at differentiation-specific promoters and for stabilizing E2f2-Rbf complexes during S phase. During development, the complex represses transcription of developmentally controlled E2f target genes. During oogenesis, plays a role in restricting DNA synthesis to sites of chorion gene amplification in late stage ovarian follicle cells. Plays an inhibitory role in ionizing radiation (IR)-induced p53-independent apoptosis. May be involved in cell cycle exit by temporarily limiting CycE-dependent activation of E2f-regulated transcription.

Gene References into Functions

E2F directly regulates the expression of late myogenic genes, with E2f1 providing the major contribution compared with E2f2. PMID: 26823289
The dE2F2 protein repress the expression of buffy, the anti-apoptotic member of the Bcl-2 family and activate the expression of an RNA-binding protein that destabilizes diap1 in Drosophila. PMID: 25188515
Heterozygous mutation of mip120 or E2f2 suppressed the binucleate cell phenotype in the peripodial epithelium of Myb-null wing discs. PMID: 23438598
dE2F2, as well as the net E2F activity, which can be depleted by mutating the common cofactor, dDp, is inhibitory for p53-independent apoptosis. PMID: 20659447
cells containing dE2F2 require dE2F1 to either prevent, or reverse, dE2F-mediated repression PMID: 12651890
RBF2 has a unique function in repressing E2F-regulated differentiation markers and dE2F2 and RBF2 are required to regulate different sets of target genes in different tissues PMID: 16082225
Combined inactivation of dE2F1 and dE2F2 had no significant effect on cell division of Hippo pathway mutant cells. PMID: 18833298

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Subcellular Location

Nucleus.

Protein Families

E2F/DP family

Tissue Specificity

Ubiquitously expressed in eye disk.

Database Links

KEGG: dme:Dmel_CG1071

STRING: 7227.FBpp0081029

UniGene: Dm.2078

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E2f2 Antibody

Product Details

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Target Background