ESR1 Antibody

Code CSB-PA882808XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ESR1 Polyclonal antibody
Uniprot No.
Target Names
ESR1
Alternative Names
ESR1 antibody; DRN antibody; ERF089 antibody; At1g12980 antibody; F3F19.1Ethylene-responsive transcription factor ESR1 antibody; Protein DORNROSCHEN antibody; Protein ENHANCER OF SHOOT REGENERATION 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ESR1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Regulates gene expression patterns in meristems and thus modulates organ development. Required for correct embryo patterning and cotyledon organogenesis. Modulates auxin signaling pathway in early embryos. Involved in the cytokinin signaling pathway that promotes shoot regeneration. Acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways.
Gene References into Functions
  1. WIND1 directly binds the ESR1 promoter and activates tts expression. PMID: 28011694
  2. Study shows that DRN and DRNL promote cell proliferation, most probably via the G1-S transition. PMID: 27277595
  3. These results define new roles for KH-domain containing proteins with ESR1 unlinking jasmonic acid-mediated growth and defense responses. PMID: 25985302
  4. Although DRN and DRNL transgenic lines are both activated in surface cells, their promoter activity marks different cell identities. PMID: 23011125
  5. ESR1 is expressed both prior to and during shoot formation, whereas ESR2 is expressed only during shoot formation. PMID: 21600396
  6. DRN and DRNL act cell-autonomously and are co-expressed in the early globular embryo, whereas expression patterns diverge during later stages of embryogeny PMID: 21161330
  7. Drn Drnl double mutants with weak or strong drnl alleles show more highly penetrant and extreme phenotypes, including a pin-like embryo without cotyledons, confirming a high degree of functional redundancy for the two genes in embryo patterning. PMID: 17376809
  8. while overexpression of ESR1 fused with a strong repression domain, SRDX, inhibited shoot regeneration. These results suggest that ESR1 induces shoot regeneration through its transactivating ability PMID: 18771931
  9. interaction of BIM1 with DRN, involved with auxin signalling, represents a possible point of hormonal crosstalk in embryonic patterning and the first example of an interaction of components of the auxin and BR signalling pathways PMID: 18830673
  10. MP binds in vivo to two AuxRE-spanning fragments in the DRN promoter; MP is required for expression of DRN in cotyledon tips. Hence, DRN represents a direct target of MP and functions downstream of MP in cotyledon development PMID: 19369397

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Subcellular Location
Nucleus.
Protein Families
AP2/ERF transcription factor family, ERF subfamily
Tissue Specificity
Expressed in shoot apical and floral meristems, and in organ primordia. Transient expression during shoot regeneration.
Database Links

KEGG: ath:AT1G12980

STRING: 3702.AT1G12980.1

UniGene: At.28308

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