esr1 Antibody

Code CSB-PA007830XA01DIL
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Product Details

Full Product Name
Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) esr1 Polyclonal antibody
Uniprot No.
Target Names
esr1
Alternative Names
esr1 antibody; esr antibody; nr3a1Estrogen receptor antibody; ER antibody; ER-alpha antibody; Estradiol receptor antibody; Nuclear receptor subfamily 3 group A member 1 antibody
Raised in
Rabbit
Species Reactivity
Danio rerio (Zebrafish) (Brachydanio rerio)
Immunogen
Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) esr1 protein
Immunogen Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
Gene References into Functions
  1. Comprehensive genetic analysis for differential functions of esr1, esr2a, and esr2b in fish reproduction. PMID: 28398516
  2. This study revealed similar patterns of transcript abundance across reproductive morphs for ERbeta1, ERbeta2, ERalpha, and aromatase in the forebrain and saccule. PMID: 23460422
  3. It was concluded that morpholino (MO) oligonucleotid technology in zebrafish embryos is an good approach for investigating the interplay of estrogen receptor subtypes in a true physiological context. PMID: 23928376
  4. Report stable reporter gene assays based on stable expression of subtypes of zebrafish ER (zfERalpha, zfERbeta1, and zfERbeta2) coupled to estrogen response element-driven luciferase in a zebrafish liver cell line. PMID: 22045033
  5. during embryogenesis two of the three 17beta-estradiol receptor genes, esr1 and esr2b are expressed, and in presence of ligand the mRNA levels of these two genes increase PMID: 20300630
  6. Data show that temperature and photoperiod significantly influence the transcription of the estrogen-responsive genes, Vtg1, Vtg2, ER alpha and ER beta after a 21-day exposure to endocrine-disrupting chemicals. PMID: 19931644
  7. Cloning of the cDNAs corresponding to three oestrogen receptors (esr1, ERalpha; esr2b, ERbeta1; and esr2a, ERbeta2 ). PMID: 12063182
  8. Data show that the hepatic expression of estrogen receptor alpha, beta1 and beta2 genes responds differently to estradiol. PMID: 15171726
  9. genistein binds and activates the three zebrafish estrogen receptors ERalpha, ERbeta-A and ERbeta-B and induces apoptosis in an ER-independent manner PMID: 19319186

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Subcellular Location
Nucleus.
Protein Families
Nuclear hormone receptor family, NR3 subfamily
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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