Pfkfb1 Antibody

Code CSB-PA017817XA01RA
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Product Details

Full Product Name
Rabbit anti-Rattus norvegicus (Rat) Pfkfb1 Polyclonal antibody
Uniprot No.
Target Names
Pfkfb1
Alternative Names
Pfkfb16-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 antibody; 6PF-2-K/Fru-2,6-P2ase 1 antibody; PFK/FBPase 1 antibody; 6PF-2-K/Fru-2,6-P2ase liver isozyme) [Includes: 6-phosphofructo-2-kinase antibody; EC 2.7.1.105); Fructose-2,6-bisphosphatase antibody; EC 3.1.3.46)] antibody
Raised in
Rabbit
Species Reactivity
Rattus norvegicus (Rat)
Immunogen
Recombinant Rattus norvegicus (Rat) Pfkfb1 protein
Immunogen Species
Rattus norvegicus (Rat)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Synthesis and degradation of fructose 2,6-bisphosphate.
Gene References into Functions
  1. Data suggest that a major portion of intracellular glucose in cardiac myocytes is apportioned for ancillary biosynthetic reactions; PFKFB1 coordinates activities of pentose phosphate, hexosamine biosynthetic, and fatty acid/glycerolipid synthesis pathways by directly modulating glycolytic intermediate entry into auxiliary glucose metabolism pathways and by indirectly regulating mitochondrial cataplerosis. PMID: 28706006
  2. Ca(2+) affects conformation of the catalytic loop 52-72 of muscle FBPase and inhibits its activity by competing with activatory divalent cations, e.g. Mg(2+) and Zn(2+). PMID: 24146906
  3. a new mRNA variant which are transcribed from a new Pfkfb1 gene promoter in fetal rat liver was characterized. PMID: 23291237
  4. flexible kinase interaction mode exists, suggesting dimer conformation mediated coupling of hormonal and posttranslational enzyme regulation to the metabolic response in liver PMID: 22668829
  5. Results describe structural differences between two isozymes of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from liver and testis. PMID: 12379646

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Protein Families
Phosphoglycerate mutase family
Tissue Specificity
Liver.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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