Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) fhuA Polyclonal antibody

Uniprot No.

P06971

Target Names

fhuA

Alternative Names

fhuA antibody; tonA antibody; b0150 antibody; JW0146Ferrichrome outer membrane transporter/phage receptor antibody; Ferric hydroxamate receptor antibody; Ferric hydroxamate uptake antibody; Ferrichrome-iron receptor antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) fhuA protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Involved in the uptake of iron in complex with ferrichrome, a hydroxamate-type siderophore. Binds and transports ferrichrome-iron across the outer membrane. In addition to its role in ferrichrome-iron transport, transports the antibiotic albomycin, which is a structural analog of ferrichrome, and acts as a receptor for colicin M, microcin J25 and bacteriophages T1, T5, phi80 and UC-1. The energy source, which is required for all FhuA functions except infection by phage T5, is provided by the inner membrane TonB system.

Gene References into Functions

Cor is an outer membrane (OM) lipoprotein that requires the participation of additional outer membrane proteins (OMPs) to exclude FhuA-dependent phages. PMID: 30043202
FhuA-PNIPAAm conjugates will serve as functional nanosized building blocks for applications in targeted drug delivery, self-assembly systems, functional membranes and transmembrane protein gated nanoreactors. PMID: 27614163
FhuA plug domain was a mostly unfolded structure that was able to occlude isolated FhuA beta-barrels inserted into the cell membrane. PMID: 22846061
In vitro experiments establish that FhuA-TonB interactions require sequential steps of kinetically limiting rearrangements. PMID: 15736954
during siderophore transport through FhuA, the cork domain remains within the barrel rather than being displaced PMID: 15994322
Results identify specific molecular contacts between TonB and outer membrane receptors such as FhuA that extend beyond the well-characterized Ton box. PMID: 16414071
the 3.3 angstrom resolution crystal structure of the TonB carboxyl-terminal domain in complex with FhuA PMID: 16741125
TonB binding promotes conformational changes in outer surface-exposed loops of FhuA. PMID: 18653801

Hide All

Subcellular Location

Cell outer membrane; Multi-pass membrane protein.

Protein Families

TonB-dependent receptor family

Database Links

KEGG: ecj:JW0146

STRING: 316385.ECDH10B_0130

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fhuA Antibody

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