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Recombinant Escherichia coli Ferrichrome-iron receptor (fhuA), partial

Recombinant Escherichia coli Ferrichrome-iron receptor (fhuA), partial

Code	CSB-YP362005ENV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP362005ENV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP362005ENV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP362005ENV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP362005ENV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

fhuA

Uniprot No.

P06971

Alternative Names

fhuA; tonA; b0150; JW0146Ferrichrome outer membrane transporter/phage receptor; Ferric hydroxamate receptor; Ferric hydroxamate uptake; Ferrichrome-iron receptor

Species

Escherichia coli (strain K12)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Involved in the uptake of iron in complex with ferrichrome, a hydroxamate-type siderophore. Binds and transports ferrichrome-iron across the outer membrane. In addition to its role in ferrichrome-iron transport, transports the antibiotic albomycin, which is a structural analog of ferrichrome, and acts as a receptor for colicin M, microcin J25 and bacteriophages T1, T5, phi80 and UC-1. The energy source, which is required for all FhuA functions except infection by phage T5, is provided by the inner membrane TonB system.

Gene References into Functions

Cor is an outer membrane (OM) lipoprotein that requires the participation of additional outer membrane proteins (OMPs) to exclude FhuA-dependent phages. PMID: 30043202
FhuA-PNIPAAm conjugates will serve as functional nanosized building blocks for applications in targeted drug delivery, self-assembly systems, functional membranes and transmembrane protein gated nanoreactors. PMID: 27614163
FhuA plug domain was a mostly unfolded structure that was able to occlude isolated FhuA beta-barrels inserted into the cell membrane. PMID: 22846061
In vitro experiments establish that FhuA-TonB interactions require sequential steps of kinetically limiting rearrangements. PMID: 15736954
during siderophore transport through FhuA, the cork domain remains within the barrel rather than being displaced PMID: 15994322
Results identify specific molecular contacts between TonB and outer membrane receptors such as FhuA that extend beyond the well-characterized Ton box. PMID: 16414071
the 3.3 angstrom resolution crystal structure of the TonB carboxyl-terminal domain in complex with FhuA PMID: 16741125
TonB binding promotes conformational changes in outer surface-exposed loops of FhuA. PMID: 18653801