Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AMP1 Polyclonal antibody

Uniprot No.

Q9M1S8

Target Names

AMP1

Alternative Names

AMP1 antibody; COP2 antibody; HPT antibody; MFO1 antibody; PT antibody; At3g54720 antibody; T5N23_80 antibody; Probable glutamate carboxypeptidase AMP1 antibody; EC 3.4.17.21 antibody; Probable glutamate carboxypeptidase 2 antibody; Probable glutamate carboxypeptidase II antibody; Protein ALTERED MERISTEM PROGRAM 1 antibody; AtAMP1 antibody; Protein CONSTITUTIVE MORPHOGENESIS 2 antibody; Protein HAUPTLING antibody; Protein MULTIFOLIA antibody; Protein PRIMORDIA TIMING antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana AMP1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

May modulate the level of one or more small signaling molecules that have a role in regulating meristem function. May play a role in balancing and restricting the meristem-promoting activity of auxin signaling. Involved in ethylene and giberellin (GA) signaling pathways or in a parallel pathway controlling cell and hypocotyl elongation and cellular organization. Involved in abscisic acid (ABA) signaling pathway. Plays a negative role in ABA-mediated seed germination and seedling development. Acts in association with LAMP1 to suppress ectopic stem cell niche formation in the shoot apical meristem (SAM) independently of cytokinin signaling pathway. Modulates responses to ABA, oxidative stress and abotic stress. Acts as negative regulator of the ABA signaling pathway to modulate freezing and drought stress responses. Mediates carbon and amino acid metabolism. May be involved in the acquisition and/or maintenance of seed dormancy. Involved in the regulation of response to heat shock and plant defense.

Gene References into Functions

Hyperphyllin-treated wild-type plants showed amp1-related tissue-specific changes of various marker genes and a significant transcriptomic overlap with the mutant. PMID: 27208298
Taken together, perturbation of CK- and ABA-related events by AMP1 mutation caused aberrant development phenotype and conflicting responses against abiotic and biotic stresses in Arabidopsis. PMID: 27743891
De novo formation of supernumerary stem cell pools was enhanced in plants mutated in both AMP1 and its paralog LIKE AMP1, indicating that they exhibit partially overlapping roles to suppress stem cell niches respecification in the peripheral zone. PMID: 25673776
Loss of ALTERED MERISTEM PROGRAM 1 function leads to supernumerary egg cells at the expense of synergids, enabling the generation of dizygotic twins. If twin embryos are formed, endosperm formation is prevented, eventually resulting in ovule abortion. PMID: 25544612
AMP1 mediates abscisic acid, oxidative and abiotic stress responses and is involved in carbon and amino acid metabolism in Arabidopsis. PMID: 23621575
report that ALTERED MERISTEM PROGRAM1 (AMP1), a gene that impacts multiple developmental processes in Arabidopsis via an unknown mechanism and its paralog LIKE AMP1 (LAMP1, At5g19740) mediate the translation inhibition, but not the RNA cleavage activity, of plant miRNAs. PMID: 23622241
There are accession-specific effects of mutations in AMP1. PMID: 21637772
auxin-derived positional information through MP carves out meristematic niches by locally overcoming a general differentiation-promoting activity involving AMP1. PMID: 17553903
identified the mfo1 mutant that produces more leaves than the wild type and bolts at the same time as the wild type; gene was cloned and found to encode a putative glutamate carboxypeptidase, the previously reported AMP1 gene. PMID: 19597310

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Subcellular Location

Endoplasmic reticulum membrane; Single-pass type II membrane protein.

Protein Families

Peptidase M28 family, M28B subfamily

Tissue Specificity

Expressed in all plant parts. Highest levels in the bolt stem, inflorescence, root and silique. Low level in leaves.

Database Links

KEGG: ath:AT3G54720

STRING: 3702.AT3G54720.1

UniGene: At.1213

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AMP1 Antibody

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